论文部分内容阅读
为探讨水杨酸(SA)对杏花抗寒性的影响机制,以早熟品种‘骆驼黄’杏的显蕾期花枝为试材,分析–2℃的低温下适宜浓度SA及其抑制剂ABT和PAC对杏花MDA、抗氧化酶和CBF转录因子的影响。结果表明,–2℃低温条件下,对照和2个SA抑制剂处理的杏花细胞膜系统均受到严重伤害,CAT、POD和SOD等抗氧化酶活性降低,MDA含量明显升高。而SA预处理的杏花在低温胁迫期间抗氧化酶活性增强,MDA含量比对照和抑制剂处理的有明显降低且相对稳定。通过荧光定量检测CBF转录因子的表达水平,表明SA能诱导杏花CBF基因的表达,尤其在低温处理3 h时,SA预处理的杏花中CBF的表达量明显高于对照和SA抑制剂处理。由此认为,适宜浓度的外源SA可能是通过调控低温下杏花中CBF转录因子的表达、增强细胞的抗氧化酶活性,减轻低温造成的膜脂过氧化伤害,从而在一定程度上增强了杏花的抗寒性。
In order to investigate the mechanism of salicylic acid (SA) on the cold resistance of apricot flowers, we used the flowering buds of the early-maturing cultivar ’camel’ Huang apricot as test material, and analyzed the appropriate concentration of SA at -2 ℃ and its inhibitor ABT And PAC on apricot MDA, antioxidant enzymes and CBF transcription factor. The results showed that under the low temperature of -2 ℃, the cell membrane system of almond flower treated by the control and two SA inhibitors were severely damaged, the activities of CAT, POD and SOD were decreased and the content of MDA was obviously increased. The SA pretreatment of apricot flower during low temperature stress increased antioxidant enzyme activity, MDA content than the control and inhibitor treatment significantly reduced and relatively stable. The expression level of CBF transcription factor was detected by fluorescence quantitative analysis, indicating that SA can induce the expression of CBF gene in apricot, especially at 3 h after cold treatment, the expression of CBF in SA pre-treated almond is obviously higher than that of control and SA inhibitor . Therefore, exogenous SA at a proper concentration may be enhanced to a certain extent by regulating the expression of CBF transcription factor in apricot flower at low temperature, enhancing the activity of antioxidant enzymes and reducing the lipid peroxidation damage induced by hypothermia Apricot cold hardiness.