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目的探究脑胶质瘤患者外周血中Th17、Th22细胞的表达及意义。方法取34例脑胶质瘤患者及24例健康对照者为研究对象,用流式细胞术检测外周血中Th17、Th22细胞的表达比例。用实时荧光定量逆转录聚合酶链反应(RT-PCR)技术检测外周血单个核细胞中视黄酸相关孤儿核受体(RORC)mRNA及芳香烃受体(AHR)mRNA的表达。用酶联免疫吸附分析(ELISA)检测血浆中Th22细胞相关因子白细胞介素-22(IL-22)的水平。结果与健康对照组相比,脑胶质瘤患者外周血Th17细胞比例(占淋巴细胞)升高不明显(P>0.05),且高级别组(WHOⅢ-Ⅳ级)与低级别组(WHOⅠ-Ⅱ级)之间差异不明显(P>0.05);Th22细胞比例(占淋巴细胞)明显升高(P<0.05),且不同级别之间差异明显(P<0.05)。脑胶质瘤患者Th17与Th22细胞呈正相关(r=0.40,P=0.03),健康对照组中二者无相关(P>0.05)。与健康对照组比较,患者外周血中RORC mRNA及AHR mRNA的升高不明显(P>0.05,P>0.05),且不同级别间差异不明显(P>0.05,P>0.05)。患者血浆IL-22水平比健康对照组明显升高(P<0.05),但不同级别之间差异不明显(P>0.05)。结论脑胶质瘤患者外周血中存在Th22细胞及IL-22异常升高,Th17细胞与Th22细胞可能共同参与胶质瘤的发生发展过程。
Objective To investigate the expression and significance of Th17 and Th22 cells in peripheral blood of patients with glioma. Methods Thirty-four patients with glioma and 24 healthy controls were enrolled in this study. Flow cytometry was used to detect the expression of Th17 and Th22 in peripheral blood. The expression of retinoic acid-related orphan nuclear receptor (RORC) mRNA and aromatic hydrocarbon receptor (AHR) mRNA in peripheral blood mononuclear cells were detected by real-time fluorescence quantitative reverse transcription polymerase chain reaction (RT-PCR) Plasma levels of Th22-related factor interleukin-22 (IL-22) were measured by enzyme-linked immunosorbent assay (ELISA). Results Compared with healthy control group, the proportion of Th17 cells in lymphocytes in peripheral blood of glioma patients was not significantly increased (P> 0.05). Compared with the control group, the levels of Th17 cells (WHO Ⅲ-Ⅳ) (P> 0.05). The proportion of Th22 cells (accounted for lymphocytes) was significantly higher (P <0.05), and the difference between different grades was significant (P <0.05). There was a positive correlation between Th17 and Th22 cells in glioma patients (r = 0.40, P = 0.03). There was no correlation between Th17 and healthy volunteers (P> 0.05). Compared with healthy control group, RORC mRNA and AHR mRNA in peripheral blood of patients had no obvious increase (P> 0.05, P> 0.05), and there was no significant difference between different levels (P> 0.05, P> 0.05). The level of plasma IL-22 in patients was significantly higher than that in healthy controls (P <0.05), but there was no significant difference between different levels (P> 0.05). Conclusion Th22 cells and IL-22 are abnormally elevated in the peripheral blood of patients with glioma, and Th17 cells and Th22 cells may participate in the development and progression of glioma.