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目的:探讨内脏脂肪素(visfatin)在胃癌中的表达及意义以及与p53的关系。方法:采用免疫组织化学法检测68例胃癌及其癌旁正常胃黏膜组织中visfatin和突变型p53的表达,分析visfatin的表达与患者临床病理特征和p53的关系,以及visfatin与p53的表达对患者生存期的影响。分别用免疫荧光和Western blot方法检测visfatin在不同人胃癌细胞株及正常胃黏膜细胞中的表达;用visfatin特异性抑制剂FK866干预胃癌细胞后,检测细胞增殖、克隆形成能力及p53蛋白的变化。结果:胃癌组织中visfatin与p53的阳性表达率均较正常胃黏膜组织明显升高(均P<0.05),两者表达均与浸润深度、淋巴结转移和TNM分期有关(均P<0.05),且visfatin与p53在胃癌组织中的表达呈明显正相关(r=0.404,P=0.001);visfatin与p53阳性表达患者中位生存时间较各自阴性表达患者明显缩短(均P<0.05),联合visfatin与p53分析显示,两者均阴性患者、两者之一阳性患者、均阳性患者的中位生存时间依次降低(χ2=15.83,P=0.000)。细胞免疫荧光和Western blot结果均显示,visfatin在不同胃癌细胞株中表达均不同程度高于正常胃黏膜细胞,其中BGC823细胞表达量最高(均P<0.05);用FK866处理后,BGC823细胞增殖与克隆形成能力明显降低,p53蛋白表达明显下调(均P<0.05)。结论:visfatin在胃癌组织中表达增高且可能与p53协同发挥促肿瘤进展的作用。
Objective: To investigate the expression and significance of visfatin in gastric cancer and its relationship with p53. Methods: Immunohistochemistry was used to detect the expression of visfatin and mutant p53 in 68 cases of gastric cancer and adjacent normal gastric mucosa. The relationship between the expression of visfatin and the clinicopathological features and p53 was analyzed, and the expression of visfatin and p53 in the patients. The impact of survival. Immunofluorescence and Western blot methods were used to detect the expression of visfatin in different human gastric cancer cell lines and normal gastric mucosa cells. After intervention of gastric cancer cells with visfatin specific inhibitor FK866, cell proliferation, colony formation and p53 protein were detected. Results: The positive rates of visfatin and p53 in gastric cancer tissues were significantly higher than those in normal gastric mucosa (all P<0.05). Both expressions were related to infiltration depth, lymph node metastasis, and TNM stage (all P<0.05). The positive expression of visfatin and p53 was significantly positive in gastric cancer tissues (r=0.404, P=0.001). The median survival time of patients with visfatin and p53 expression was significantly shorter than that of negative expression (all P<0.05). The p53 analysis showed that the median survival time of both negative patients, positive patients and positive patients decreased sequentially (χ2=15.83, P=0.000). The results of immunofluorescence and Western blot showed that visfatin expression was higher in different gastric cancer cell lines than in normal gastric mucosa cells, and BGC823 cells had the highest expression levels (all P<0.05). The proliferation of BGC823 cells after treated with FK866. The ability of colony formation was significantly reduced, and the expression of p53 protein was significantly down-regulated (all P<0.05). Conclusion: The expression of visfatin is increased in gastric cancer tissues and may play a role in promoting tumor progression in concert with p53.