对三个甘蓝型油菜隐性细胞核雄性不育系花药发育的解剖学观察表明，S45A和117A小孢子发生的细胞学特点相同，其雄性败育于减数分裂后的四分体至单核小孢子阶段。其主要特点是减数分裂完成后，小孢子不形成花粉壁并开始解体；同时，绒毡层细胞径向扩大和小液泡化并逐渐解体。9012A雄性败育于花粉母细胞至减数分裂四分体阶段。其主要特点是花粉母细胞减数分裂后胼胝质壁不降解和不释放出四分体，并且四分体开始液泡化解体；同时，绒毡层细胞逐步大液泡化，直至最后解体。 Anatomical observation of anther development of three recessive nuclear male sterile lines in Brassica napus showed that the cytological characteristics of the microspores of S45A and 117A were the same. The male was aborted in meiosis quartile to mononuclear Spore stage. Its main feature is that after the meiosis is completed, the microspores do not form pollen wall and begin to disintegrate. At the same time, the tapetal cells expand radially and little vacuolize and disintegrate gradually. 9012A male was aborted in pollen mother cells to meiosis quartile. Its main feature is the pollen mother cell meiosis does not degrade the corpus callosum wall and does not release the tetrad, and the tetrad began vacuolar disintegration; the same time, tapetal cells gradually vacuolar until finally disintegrated.