在常规选育根瘤菌的程序中,由于菌种的分离、纯化工作量大,鉴定技术的精确度不高,致使大量筛选菌种受到限制。用根瘤固氮酶活性作为预测筛选根瘤菌的有效性指标,可简化育种程序,提高选种效率。1977年至1979年,我们以稻区冬绿肥紫云英根瘤菌为对象,利用乙炔还原法,进行快速筛选根瘤菌的研究。此法是通过试管幼苗法的感染性和有效性的鉴定,然后分 In the routine selection of rhizobia, the strain of large number of strains was limited due to the large amount of isolation and purification of the strains and the lack of accuracy of the identification technique. Using nodule nitrogenase activity as a predictor of the effectiveness of screening rhizobia can simplify breeding programs and improve selection efficiency. From 1977 to 1979, we used the acetylene reduction method for the rapid screening of Rhizobium strains in the winter green manure (Astragalus sinicus) in the rice area. This method is through in vitro seedling method of infectivity and effectiveness of identification, and then points