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在自然杀细胞试验中广泛用作靶细胞的K562细胞系,是1975年Lozzio等由慢性粒细胞型白血病人急变期培育出的。经Klein(1976)等研究将其定为粒细胞系,因为它有Ph~1染色体,而且来自慢粒病人。Greaves等(1977)提出慢粒急变期细胞往往由淋巴系细胞来;但是K562细胞并无任何淋巴细胞特征,所以仍认为属于未分化的粒细胞系。作者根据下列实验结果认为K562细胞系来源于红细胞克隆: 首先,K562细胞的表面糖蛋白与良性和恶性的粒细胞表面糖蛋白十分不同,与淋巴细胞系的也不同,而且有正常红细胞膜上的许多标记蛋白。作者将被测细胞的表面糖蛋白用半乳糖过氧化物酶-
The K562 cell line, which has been widely used as a target cell in a natural killer cell test, was developed in 1975 by Lozzio et al. from the human blast crisis of chronic myeloid leukemia. Klein (1976) and other studies identified it as a granulocyte line because it has a Ph~1 chromosome and is from a patient with chronic granulopathy. Greaves et al. (1977) suggested that cells in the CRM period are often derived from lymphoid cells; however, K562 cells do not have any lymphocyte characteristics and are therefore considered to be undifferentiated granulocyte lines. Based on the following experimental results, the authors conclude that the K562 cell line is derived from the red cell clone: First, the surface glycoprotein of K562 cells is very different from the benign and malignant granulocyte surface glycoproteins, and also different from the lymphocyte lineages, and there are also normal erythrocyte membranes. Many marker proteins. The authors will test the surface glycoproteins of cells using galactose peroxidase-