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1973年Latt等相继发现,在含有5—溴脱氧尿嘧啶核苷酸(5—Bromidoxyuridine,Budr)的培养基中,生长两个周期的细胞,进行分化染色时,在姐妹染色单体之间显出深浅不同的颜色,可用以检测姐妹染色单体互换情况。1975年Latt;Parry和Evans等再次报导了改进和发展起来的SCE技术,对于检测可疑的诱变剂或致癌剂是一个高度灵敏的指示器。各种已知的诱变剂或致癌剂,即使其浓度在细胞毒理学和染色体断裂水平以下时,也能诱发SCE;从而SCE试验在致
In 1973, Latt et al. successively discovered that in the medium containing 5-bromodioxyuridine (Budr), two cycles of cells were grown and differentiated between sister chromatids when differentiated and stained. Different shades of color can be used to detect sister chromatid exchanges. In 1975, Latt; Parry and Evans et al. once again reported that the improved and developed SCE technology is a highly sensitive indicator for the detection of suspected mutagens or carcinogens. Various known mutagens or carcinogens can induce SCE even if the concentration is below the level of cytotoxicity and chromosomal breakage; thus the SCE test results in