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甲胎蛋白(AFP)是一种癌胚蛋白。它可在胚胎发育过程中或在恶性肿瘤如肝癌、性腺的生殖细胞肿瘤中出现。在胚胎时期,AFP是由卵黄囊或胚肝合成的.利用具有不同活性的抗ConA或LCH可将卵黄囊或胚肝产生的A FP区分开来。因此,作者利用这一性质分析了34例患有原发性肝癌和4例胃癌肝转移病人血清中AFP的亚组分,业利用ConA或LCH交联的免疫亲和层析将A FP进行分离. 结果表明,利用ConA可发现胚肝产生的亚组分一般只在原发性肝癌和转移性肝癌中出现,而卵黄囊产生的亚组分在34例原发性肝癌中只有7例(20.6男)出现.然而在4例转移性肝癌中全部被检测出来(100多)。若利用LCH分离AFP,胚肝产生的亚组分在原发性肝癌和转移性肝癌中都存在,而卵黄囊产生的亚组分只在转移性肝癌中出现. 鉴于上述结果,作者认为原发性肝癌的AFP的糖基化作用与胃癌肝转移的不同.此外,还提示肝癌和胚肝所合成的AFP的糖基化也不尽相同,而且肝癌的AFP合成不总是如在胚肝那种返祖性表达的. 在临床上,当血清中A FP水平升高时,
Alpha-fetoprotein (AFP) is an oncoprotein. It can occur during embryonic development or in malignant tumors such as liver cancer, gonadal germ cell tumors. In the embryonic stage, AFP is synthesized from the yolk sac or embryonic liver. The yolk sac or embryonic liver produced A FP can be distinguished using anti-ConA or LCH with different activities. Therefore, the authors used this property to analyze the subfractions of AFP in serum from 34 patients with primary liver cancer and 4 patients with liver metastases from gastric cancer. They were isolated by immunoaffinity chromatography using ConA or LCH cross-linked immunoaffinity chromatography. The results showed that the use of ConA can be found in sub-components of embryonic liver generally only in primary liver cancer and metastatic liver cancer, and sub-components produced by the yolk sac in 34 cases of primary liver cancer in only 7 cases (20.6 Male) appeared. However, all 4 cases of metastatic liver cancer were detected (more than 100). If LFP is used for the separation of AFP, the subfractions produced by embryonic liver are present in primary liver cancer and metastatic liver cancer, while the subfractions produced by yolk sac appear only in metastatic liver cancer. In view of the above results, the authors believe that the primary origin The glycosylation of AFP in liver cancer is different from that in gastric cancer. In addition, it also suggests that the glycosylation of AFP synthesized by liver and embryonic liver is not the same, and the AFP synthesis of liver cancer is not always the same as in embryonic liver. This is a kind of retrograde expression. Clinically, when the level of AFP in serum increases,