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目的观察32P-磷酸铬-聚L-乳酸(32 P-CP-PLLA)粒子瘤体植入后对荷H22肝癌KM小鼠移植瘤的抑瘤效果和对Caspase-3和Caspase-8蛋白表达的影响。方法建立荷H22肝癌KM模型小鼠45只后均分为粒子组(瘤内植入活度为7.4MBq的32P-CP-PLLA粒子1枚)、胶体组(瘤内注射活度为7.4MBq的32P-CP胶体)和对照组三组。分别于第1、6、12、24小时每组各处死3只小鼠,用分光光度法检测瘤体Caspase-3和Caspase-8的表达。每组3只小鼠每2天测量肿瘤体积,第14天测量肿瘤质量,计算抑瘤率。结果治疗第14天,粒子组抑瘤率高于胶体组[(62.28±2.36)%vs.(50.28±3.22)%](P<0.05)。与对照组比较,胶体组和粒子组各时间点的Caspase-3和Caspase-8的表达增高(P<0.05)。结论 32P-CP-PLLA粒子对荷H22小鼠移植瘤具有抑瘤和诱导细胞凋亡作用,适合于肝癌实体瘤的近距离治疗。
Objective To observe the antitumor effect and the expression of Caspase-3 and Caspase-8 in xenografts of KM mice bearing H22 hepatocarcinoma after 32P-chitosan-poly-L-lactic acid (32P-CP-PLLA) influences. Methods Forty-five H22 hepatocarcinoma KM mice were divided into three groups: the group of 32P-CP-PLLA with a tumor volume of 7.4MBq, and the colloid group with a tumor volume of 7.4MBq 32P-CP colloid) and control group three groups. At the 1st, 6th, 12th and 24th hour, 3 mice in each group were sacrificed, and the expression of Caspase-3 and Caspase-8 were detected by spectrophotometry. Tumor volume was measured every 2 days in 3 mice in each group, tumor mass was measured on the 14th day, and tumor inhibition rate was calculated. Results On the 14th day of treatment, the tumor inhibition rate in the particle group was higher than that in the colloidal group [(62.28 ± 2.36)% vs (50.28 ± 3.22)%] (P <0.05). Compared with the control group, the expression of Caspase-3 and Caspase-8 at colloidal group and particle group increased at each time point (P <0.05). Conclusion 32P-CP-PLLA particles can inhibit tumor growth and induce apoptosis in H22 mouse xenografts and is suitable for brachytherapy of hepatocellular carcinoma.