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为了分离人白血病病毒,自1976年以来我们相继建立了粒—单型人白血病细胞系J_(6-1)和单核型人白血病细胞系J_(6-1)(第三系J_(6-3)已传20代,正继续培育和鉴定中)。 J_(6-1)胞来自急性粒单型白血病病人周围血白细胞,曾传64代。因初期培养时某些条件所限,曾出现四次危机,50代后明显变异,至64代不能传代而死亡。后将液氮保存的较早期培养物复苏后再传代,至
In order to isolate human leukemia virus, we have successively established the granulo-unilotype human leukemia cell line J_(6-1) and the monokaryotic human leukemia cell line J_(6-1) (tertiary line J_(6- 3) It has been passed for 20 generations and is continuing to be cultivated and appraised.) J_(6-1) cells from peripheral blood leukocytes in acute leukemia patients have been transmitted for 64 generations. Due to certain conditions during the initial training, there have been four crises, significant variations after 50 generations, and deaths that could not be passed until the 64th generation. After resuscitating the earlier cultures preserved in liquid nitrogen, they are passed to