【摘 要】
：
Tbx5参与转录因子的调控,对上肢及心脏发育起关键作用.通过对小鼠胚胎心脏组织切片厚度的选择、加入抗原修复液等试验条件的优化,建立了利用切片免疫荧光技术检测小鼠胚胎心
【机 构】
：
湖南师范大学蛋白质化学及鱼类发育生物学教育部重点实验室心脏发育研究中心,
论文部分内容阅读
Tbx5参与转录因子的调控,对上肢及心脏发育起关键作用.通过对小鼠胚胎心脏组织切片厚度的选择、加入抗原修复液等试验条件的优化,建立了利用切片免疫荧光技术检测小鼠胚胎心脏中Tbx5的方法.结果显示,在胚胎发育的第11.5 d,Tbx5在整个心脏中都有表达,尤其在心房以及左心室中高表达.但在胚胎发育的第13.5 d,Tbx5只在心房和左心室中表达,在右心室中并不表达.揭示Tbx5的这种动态表达模式对于心脏的发育以及腔室分化是十分重要的.
Tbx5 is involved in the regulation of transcription factors and plays a key role in the development of the upper limbs and the heart.Through the optimization of the experimental conditions such as the selection of mouse embryonic heart tissue thickness and the addition of antigen repair solution, Tbx5 was expressed in the whole heart at embryonic day 11.5, especially in the atrium and left ventricle.However, on day 13.5 embryos, Tbx5 only expressed in the atria and left ventricle , Which is not expressed in the right ventricle, revealing that this dynamic expression pattern of Tbx5 is important for heart development and compartment differentiation.
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