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应用核酸酶Ｐ１介导的￣（３２）Ｐ后标记分析方法，分析了３２例胸科手术后切除的肺癌病人的正常肺组织中ＤＮＡ加合物。在３２例肺癌手术者中，２２例为吸烟者，１０例为非吸烟者。本实验吸烟组与非吸烟组肺组织ＤＮＡ加合物水平差异有显著性（Ｐ＜０．００１）；在吸烟者的放射自显影图片上出现一条斜的放射带。相关性分析表明，随着吸烟量的增加，肺组织中ＤＮＡ加合物也增加，有显著相关（ｒ＝０．７３，Ｙ＝０．４１１Ｘ＋３．５２，Ｐ＜０．０１）。
Using a nuclease P1 mediated (32)P post-labeling analysis method, DNA adducts in the normal lung tissue of 32 lung cancer patients resected after thoracic surgery were analyzed. Of the 32 lung cancer patients, 22 were smokers and 10 were non-smokers. There was a significant difference in the level of DNA adducts in lung tissue between the smoking and non-smoking groups in this study (P < 0.001); an oblique radiation band appeared on the autoradiogram of smokers. The correlation analysis showed that with the increase of smoking, DNA adducts in lung tissue also increased, there was a significant correlation (r=0.73, Y=0.411X+3.52, P<0.01).