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目的:观察雷帕霉素(RA-PA)对不同PTEN表达子宫内膜癌裸鼠移植瘤的抑制作用。方法:通过慢病毒转染构建稳定表达绿色荧光蛋白(GFP)的HEC-1A(PTEN阳性)和Ishikawa(PTEN阴性)细胞株,建立裸鼠移植瘤模型。活体成像系统观察肿瘤的生长情况。观察RAPA治疗后肿瘤的体积及重量的变化。HE染色观察肿瘤的病理形态学变化。结果:建立稳定表达GFP的子宫内膜癌细胞系及裸鼠移植瘤模型,活体荧光成像显示,治疗组裸鼠荧光强度较对照组明显减弱。治疗组肿瘤体积及重量明显小于对照组(P<0.05),Ishikawa细胞组的抑瘤率(67.1%)较高于HEC-1A细胞组的抑瘤率(48.1%)。治疗组的肿瘤组织可见大片肿瘤细胞坏死,对照组肿瘤细胞坏死少。结论:RAPA对PTEN阳性及阴性的子宫内膜癌生长均有明显的抑制作用,PTEN的丢失可增加RAPA抗子宫内膜癌的敏感性。
Objective: To observe the inhibitory effect of rapamycin (RA-PA) on different PTEN-expressing xenografts in nude mice. Methods: The HEC-1A (PTEN positive) and Ishikawa (PTEN negative) cell lines stably expressing green fluorescent protein (GFP) were constructed by lentivirus transfection to establish a nude mouse xenograft model. Live imaging system to observe the growth of the tumor. Observe the tumor volume and weight changes after RAPA treatment. The pathological changes of the tumor were observed by HE staining. Results: The endometrial carcinoma cell line stably expressing GFP and the nude mouse xenograft model were established. Fluorescence imaging showed that the fluorescence intensity of nude mice in treatment group was significantly weaker than that in control group. The tumor volume and weight in the treatment group were significantly lower than those in the control group (P <0.05). The inhibition rate of Ishikawa cells (67.1%) was higher than that of HEC-1A cells (48.1%). The tumor tissue of the treatment group showed large tumor cell necrosis, and the tumor cell necrosis of the control group was small. CONCLUSION: RAPA can significantly inhibit the growth of PTEN-positive and -negative endometrial carcinomas. The loss of PTEN increases the sensitivity of RAPA to endometrial cancer.