论文部分内容阅读
Objective To establish an HPLC method for the determination of hederagenin and oleanolic acid in Clematidis Radix et Rhizoma.Method Using Waters XbridgeTM-C18 column (250 mm×4.6 mm,5 μm) at 25 ℃,acetonitrile-water (90∶ 10) as the mobile phase,the HPLC analysis was performed with flow rate of 1.0 mL/min.and PDA detection wavelength at 205 nm.Result The hederagenin showed a good linear relationship between 2.008-25.100 ng/mL (r2=0.999 8),and the oleanolic acid assumed a good linear relationship between 2.238-22.380 ng/mL (r2=0.999 1).The content of hederagenin in Clematidis Radix et Rhizoma from different areas could satisfy the quantitative specification of Chinese Pharmacopoeia,while that of oleanolic acid does not.Conclusion The contents of bioactive compositions of Clematidis Radix et Rhizoma vary significantly among different producing areas,on the aspect of quantitation of the chemical markers.
Objective To establish an HPLC method for the determination of hederagenin and oleanolic acid in Clematidis Radix et Rhizoma. Method using Waters Xbridge ™ -C18 column (250 mm × 4.6 mm, 5 μm) at 25 ° C., acetonitrile-water (90:10) as the mobile phase, the HPLC analysis was performed with flow rate of 1.0 mL / min. and PDA detection wavelength at 205 nm. Result The hederagenin showed a good linear relationship between 2.008-25.100 ng / mL (r2 = 0.999 8), and the oleanolic acid assumed a good linear relationship between 2.238-22.380 ng / mL (r2 = 0.999 1). The content of hederagenin in Clematidis Radix et Rhizoma from different areas could satisfy the quantitative specification of Chinese Pharmacopoeia, while that of oleanolic acid does not. Conclusion The contents of bioactive compositions of Clematidis Radix et Rhizoma vary significantly among different producing areas, on the aspect of quantitation of the chemical markers.