通常采用电子计算机断层扫描法(CT),来判断腹膜后的淋巴结有无转移性肿瘤,淋巴结大于1.5cm或粘连成团时,认为是异常。但是,CT不能确定淋巴结的内部特征,当淋巴结为正常大小时,就不能确定淋巴结是否有恶性改变。作者应用小鼠单克隆抗体1083—17—1A(IgG_2a),用胃蛋白酶裂解成无菌、无热原质的F(ab′)_2片段,标志~(131)I后,静脉注入结肠癌患者的体内,通过放射性核素显像,来判断有无腹膜后淋巴结转移。一例在肾脏和左肾门水平的中线附近显像。外科医生根据单克隆抗体扫描结果,切除了最大可触及的淋巴结,直径不到1cm。冰冻切片和石蜡切片病理学检查呈现一些腺癌微小病灶,表明为原发性结肠癌的转移,用免疫过氧化物酶对1083—17—1A结肠直肠癌抗 Computed tomography (CT) is commonly used to determine whether retroperitoneal lymph nodes have metastatic tumors. Lymph nodes are larger than 1.5 cm or adherently clustered and considered abnormal. However, CT cannot determine the internal characteristics of the lymph nodes. When the lymph nodes are of normal size, it cannot be determined whether the lymph nodes have malignant changes. The authors used mouse monoclonal antibody 1083-17-1A (IgG-2a) and was cleaved by pepsin to form a sterile, pyrogen-free F(ab’)-2 fragment. After the injection of 131I, the patient was injected intravenously with colon cancer. In vivo, radionuclide imaging was used to determine the presence or absence of retroperitoneal lymph node metastasis. One case was seen near the midline of the kidney and the level of the left kidney gate. According to the results of the monoclonal antibody scan, the surgeon removed the most palpable lymph nodes with a diameter of less than 1 cm. Pathological examination of frozen sections and paraffin sections revealed some adenocarcinoma micro-lesions, indicating the metastasis of primary colon cancer, using immunoperoxidase to resist 1083-17-1A colorectal cancer