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本文报告在尼日利亚进行的一次回顾性登革免疫的血清学调查,旨在确定这类病毒感染在人和非人的灵长类中的流行。初步的血凝抑制试验揭示63%的受检人有以下一种或多种的黄病毒血抑抗体:登革1型,黄热病、西尼罗及 Wesselsbron。根据对179份血清作平行的血抑及中和试验,显示20份黄病毒血抑抗体阴性血清中有6份(30%)台登革中和抗体。这个数据强调了在筛选登革病毒免疫时,中和试验的优点胜于血抑。采自尼日利亚不同地理景观的1,816份人血清进行中和试验,证明45%的尼日利亚人对登革2型病毒有免疫。免疫的百分比在20岁以上的成人(51%)明显高于儿童(37%)(P<0.01)。在所有四个生态地带的标本中,观察到以次生草原带的登革中和抗体百分率最高(63%);其次为雨林带(42%)。南几内亚草原和高原带中人的登革免疫百分率较低。血清流行病学抗体调查,以城市人群的抗体(48%)高于农村人群(37%)。在登革免疫血清试验中,显示35%的血清仅含登革或登革同另一种病毒的中和抗体。因此,这种登革免疫者不可能当作黄病毒组内的异种交叉反应。此外,在猴和(犭互)(galago)中找到了登革感染的证据,即48%的猴及25%的含登革中和抗体。一些血清中存在特异性登革中和抗体,提示在尼日利亚发生森林登革热循环是可能的。
This article reports a serological investigation of retrospective dengue immunization in Nigeria to determine the prevalence of such viral infections in human and non-human primates. The initial hemagglutination inhibition test revealed that 63% of the subjects had one or more of the following flavivirus antibodies: Dengue Type 1, Yellow Fever, West Nile and Wesselsbron. Based on a parallel blood and neutralization test of 179 sera, six (30%) of the dengue neutralizing antibodies were found in 20 of the flaviviridae negative sera. This data underscores the advantage of the neutralization test over blood suppression when screening for dengue virus immunity. A neutralization test of 1,816 human sera collected from different geographies in Nigeria demonstrated that 45% of Nigerians are immunized against Dengue 2 virus. The percentage of immunized adults over the age of 20 (51%) was significantly higher than that of children (37%) (P <0.01). Among all four ecoregions, the highest percentage of dengue neutralizing antibodies (63%) was observed in the secondary grassland belt, followed by the rain forest zone (42%). The lower the percentage of dengue immunization in South Guinea grassland and plateau zone. Serological epidemiological antibody surveys to urban populations (48%) were higher than in rural areas (37%). In dengue immune serum tests, 35% of sera were shown to contain only neutralizing antibodies to dengue or dengue to another virus. Therefore, such dengue immunization can not be regarded as cross-reactions within the flavivirus group. In addition, evidence of dengue infection was found in monkeys and galago, ie 48% of monkeys and 25% of dengue neutralizing antibodies. The presence of specific dengue neutralizing antibodies in some sera suggests that dengue cycling in forests is possible.