The multiwalled carbon nanotubes thin-film-based electrode was fabricated by electrophoretic deposition and modified with copper(Cu) nanoparticles to fabricate Cu/CNTs nanocomposite sensor for nonenzymatic glucose detection.The expensive glassy carbon electrode was replaced by fluorine-doped tin oxide glass containing CNTs film to confine the Cu nanoparticles growth by electrodeposition through cyclic voltammetry(CV). The ultraviolet visible and X-ray diffraction analysis revealed the successful deposition of Cu nanoparticles on the CNTs-modified electrode. The atomic force microscopy images confirmed the morphology of electrodeposited Cu on CNTs film as uniformly dispersed particles.The electrocatalytic activity of electrode to the glucose oxidation was investigated in alkaline medium by CV and amperometric measurements. The fabricated sensor exhibited a fast response time of less than 5 s and the sensitivity of 314μA mM~(-1)cm~(-2)with linear concentration range(0.02–3.0 mM) having detection limit 10.0μM. Due to simple preparation of sensor, Cu/CNTs nanocomposite electrodes are a suitable candidate for reliable determination of glucose with good stability. The multiwalled carbon nanotubes thin-film-based electrode was fabricated by electrophoretic deposition and modified with copper (Cu) nanoparticles to fabricate Cu / CNTs nanocomposite sensor for nonenzymatic glucose detection. Expensive carbony electrode was replaced by fluorine-doped tin oxide glass containing CNTs film to confine the Cu nanoparticles growth by electrodeposition through cyclic voltammetry (CV). The ultraviolet visible and X-ray diffraction analysis revealed the successful deposition of Cu nanoparticles on the CNTs-modified electrode. The atomic force microscopy confirmed the morphology of electrodeposited Cu on CNTs film as locally dispersed particles. The electrocatalytic activity of electrode to the glucose oxidation was investigated in alkaline medium by CV and amperometric measurements. The fabricated sensor showed a fast response time of less than 5 s and the sensitivity of 314 μA mM ~ ( -1) cm ~ (-2) with linear concentration range (0.02-3.0 mM) havin g detection limit 10.0 μM. Due to simple preparation of sensor, Cu / CNTs nanocomposite electrodes are a suitable candidate for reliable determination of glucose with good stability.