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本试验采用了早春播种,让其植株在冷凉的气候条件下开花,并结合蕾期授粉的方法。克服了自交不亲合性,获得了3000多粒IVP35(S.Phureja)的自交种子。 1984年以东农303(雄性不育)作被测母本,用IVP35各自交后代作授粉者所作的测交结果表明,诱发单倍体的隐性基因不只1对, 因为IVP35自交后代单株无性系间诱发单倍体的频率有显著差异。本试验还选育出了优良授粉者NEA—P16和NEA—P19。按100个浆果中产生孤雌生殖的植株来计算,NEA—P16和NEA—P19诱发孤雌生殖的频率分别为IVP35的2.66和2.48倍。与优良授粉者的选育及孤雌生殖有关的问题在本论文中也进行了讨论。
In this experiment, sowing was carried out in early spring, which allowed the plants to flowering under cool climatic conditions and combined with budding pollination methods. Overcame the self-incompatibility, obtained more than 3000 self-seeding seeds of IVP35 (S.Phureja). In 1984, Dongnong 303 (male sterility) was used as the tested female parent. The results of cross-fertilization with IVP35 and their offsprings as pollinators showed that there were not only one pair of recessive genes in haploid. Because IVP35 self-pollinated single There was a significant difference in the frequency of haploid induced between strains. This experiment also bred excellent pollinators NEA-P16 and NEA-P19. The number of parthenogenesis induced by NEA-P16 and NEA-P19 was calculated to be 2.66 and 2.48 times of that of IVP35, respectively, based on the production of parthenogenetic plants in 100 berries. The issues related to breeding and parthenogenesis of good pollinators are also discussed in this paper.