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甜瓜转化率低的问题仍具有挑战性。用1个新特质甜瓜自交系BU-21/3作材料研究其再生和转化能力,表明这个自交系的再生能力优于以往研究过的基因型。用含有报告基因GUS或GFP的根癌农杆菌浸染子叶外植体,研究瞬时表达和稳定表达效率,观察到了大量瞬时表达,稳定表达时每个外植体可产生0.4～1.5个转基因芽,在转化后的8～10周,将转基因植株移入可控温室,发现表型和育性正常。再生能力的遗传分析表明它受单个显性位点控制,因此,这种新特质的基因型有望成为甜瓜转基因育种的有效工具。
The problem of low melon conversion is still challenging. A new trait melon inbred line BU-21/3 was used as material to study its regeneration and transformation ability, indicating that this inbred line has better regeneration ability than the previously studied genotypes. Cotyledonary explants were infected with Agrobacterium tumefaciens containing the reporter gene GUS or GFP to investigate transient and stable expression efficiency and a large number of transient expression was observed. Stable expression yielded 0.4-1.5 transgenic buds per explant. After 8 to 10 weeks of transformation, the transgenic plants were transferred to a controllable greenhouse and the phenotype and fertility were found to be normal. Genetic analysis of regenerative ability shows that it is controlled by a single dominant site, therefore, the genotype of this new trait is expected to be an effective tool for GM melon breeding.