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为研究膜醭毕赤酵母(Pichia membranaefaciens)对杨梅果实绿霉病的抑制及其对抗病性的诱导作用,将杨梅果实先接种15μL浓度为1×109cfu/mL的膜醭毕赤酵母菌悬液,风干1h后再接种15μL的1×105cfu/mL的桔青霉(Penicillium citrinum)孢子悬浮液,然后转入(1±1)℃下贮藏8d,每隔2d测定果实腐烂率、病斑直径、抗病相关酶几丁质酶、β-1,3-葡聚糖酶、苯丙氨酸解氨酶(PAL)、过氧化物酶(POD)、多酚氧化酶(PPO)活性以及总酚含量;同时分析了离体条件下P.membranaefaciens对P.citrinum孢子萌发和芽管伸长的影响。结果显示,P.membranaefaciens可显著抑制果实贮藏期间绿霉病的发生,并可诱导几丁质酶、β-1,3-葡聚糖酶、PAL、POD和PPO活性和总酚含量的升高;同时P.membranaefaciens在离体条件下也明显抑制病原菌的生长。因此,P.membranaefaciens抑制杨梅果实采后腐烂与直接抑制病原菌生长以及间接诱导抗病性密切相关。
In order to study the inhibitory effect of Pichia membranaefaciens on the green mold of Myrica rubra and its induction of disease resistance, the bayberry fruit was first inoculated with 15 μL of Pichia pastoris suspension with a concentration of 1 × 109 cfu / mL After 1 h of air-drying, 15 μL of 1 × 105 cfu / mL Penicillium citrinum spore suspension was inoculated and then stored at (1 ± 1) ° C for 8 days. Fruit decay rate and spot diameter , Disease-related enzymes chitinase, β-1,3-glucanase, phenylalanine ammonia lyase (PAL), peroxidase (POD), polyphenol oxidase (PPO) The contents of phenol and phenol in P. citrinum were studied. The effects of P.membranaefaciens on the spore germination and tube elongation were also analyzed. The results showed that P.membranaefaciens significantly inhibited the occurrence of green mold during fruit storage and induced the increase of chitinase, β-1, 3-glucanase, PAL, POD and PPO activity and total phenolic content At the same time, P.membranaefaciens significantly inhibited the growth of pathogenic bacteria in vitro. Therefore, P. membranaefaciens inhibition of bayberry fruit decay after the direct inhibition of pathogen growth and indirectly induced resistance is closely related.