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[会议论文] 作者：Huiming Yuan,Lihua Zhang,Yukui Zhang,
来源：2016年分析化学前沿国际研讨会及中美分析化学研讨会 年份：2016
For bottom-up based proteome analysis,sample preparation including protein extraction,purication and digestion is indispensable prior to LC-MS analysis.Howe...
[会议论文] 作者：Lihua Zhang,Yu Liang,Huiming Yuan,Yukui Zhang,
来源：第2届大连国际色谱学术报告会、第37届国际高效液相色谱及相关技术会议、第18届全国色谱学术报告会 年份：2011
Proteomics has been paid much attention to discover candidate biomarkers for clinic diagnosis, understand the potential mechanisms for diseases, and screen...
Integrated platform for High Throughput Quantitative Proteome Analysis with Combination of Simultane
[会议论文] 作者：Huiming Yuan,Lihua Zhang,Yukui Zhang,Shen Zhang,
来源：第八届中国蛋白质组学大会 年份：2013
...
Fully Automated Protein Absolute Quantification Platform for Simultaneous Analysis of Multiple Tumor
[会议论文] 作者：Huiming Yuan,Yuanbo Chen,Lihua Zhang,Yukui Zhang,
来源：2018年中国质谱学术大会 年份：2018
The proteins in the circulatory system reflect the individual's physiological and disease states.However,many protein biomarkers used for clinical diagnosis...
INTEGRATED PLATFORM FOR LARGE-SCALE PROTEOME PROFILING WITH COMBINATION OF MICRO-REVERSED PHASE LIQU
[会议论文] 作者：Huiming Yuan,Zhongpeng Dai,Lihua Zhang,Yukui Zhang,
来源：第十六届北京分析测试学术报告会 年份：2015
...
Integrated platform for High Throughput Quantitative Proteome Analysis with Combination of Simultane
[会议论文] 作者：Huiming Yuan,Shen Zhang,Lihua Zhang,Yukui Zhang,
来源：第八届中国蛋白质组学大会 年份：2013
For O18 labeling based comparative proteome analysis,enzyme-catalyzed protein digestion and peptide labeling are two crucial sample preparation steps for protein identification and quantification.Howe...
INTEGRATED PLATFORM FOR LARGE-SCALE PROTEOME PROFILING WITH COMBINATION OF MICRO-REVERSED PHASE LIQU
[会议论文] 作者：Huiming Yuan,Zhongpeng Dai,Lihua Zhang,Yukui Zhang,
来源：第十六届北京分析测试学术报告会 年份：2015
For proteome analysis,two-dimensional high performance liquid chromatography coupled with tandem mass spectrometry(2D-HPLC-MS/MS)has been considered as one of the most popular approaches,by which seve...
Fully Automated Protein Absolute Quantification Platform for Simultaneous Analysis of Multiple Tumor
[会议论文] 作者：Huiming Yuan,Yuanbo Chen,Lihua Zhang,Yukui Zhang,
来源：2018年中国质谱学术大会 年份：2018
The proteins in the circulatory system reflect the individuals physiological and disease states.However,many protein biomarkers used for clinical diagnosis are present at very low concentration in bod...
[会议论文] 作者：Yuan Zhou,Huiming Yuan,Qun Zhao,Lihua Zhang,Yukui Zhang,
来源：2016年分析化学前沿国际研讨会及中美分析化学研讨会 年份：2016
Proteome quantifiation is of great signifcane to study various biological processes.In our recent work,various new methods for the relative quantification o...
PREPARATION OF SUB-2 MICRON CORE-SHELL SILICA MICROSPHERES AND APPLICATIONs IN RAPID HPLC SEPARATION
[会议论文] 作者：Lihua Zhang,Yi Min,Huiming Yuan,Kaiguang Yang,Yukui Zhang,
来源：43rd International Symposium on High Performance Liquid Phas 年份：2015
...
Integrated platform of capillary isoelectric focusing,trypsin immobilized enzyme microreactor and na
[会议论文] 作者：Tingting Wang,Huiming Yuan,Lihua Zhang,Zhen Liang,Yukui Zhang,
来源：第2届大连国际色谱学术报告会、第37届国际高效液相色谱及相关技术会议、第18届全国色谱学术报告会 年份：2011
In proteomic study, top-down and bottom-up approaches have been widely adopted.However, each approach has its own weaknesses.For example, the challenge in protein separation/fragmentation largely rest...
Integration of Protein Digestion Microreactors in a Comprehensive Two-Dimensional Liquid Chromatogra
[会议论文] 作者：Filippo Bedani,Hao Jiang,Huiming Yuan,Lihua Zhang,Yukui Zhang,
来源：第2届大连国际色谱学术报告会、第37届国际高效液相色谱及相关技术会议、第18届全国色谱学术报告会 年份：2011
Proteins are an extremely important class of (bio)macromolecules as virtually no process within cells occurs without their direct involvement.As a consequence, their analysis is of paramount relevance...
Highly efficient two dimensional nano-liquid chromatography tandem mass spectrometry platform combin
[会议论文] 作者：Simin Xia,Huiming Yuan,Zhen Liang,Lihua Zhang,Yukui Zhang,
来源：第2届大连国际色谱学术报告会、第37届国际高效液相色谱及相关技术会议、第18届全国色谱学术报告会 年份：2011
With the acceleration of proteome research, it becomes urgent to develop novel approaches for high efficient and high throughput analysis of proteins.2D nano-HPLC is one of the most prospective approa...
An Integrated Protein Pretreatment Device as the Interface of Two-dimensional High Performance Liqui
[会议论文] 作者：Huiming Yuan,Hao Jiang,Zhongpeng Dai,Lihua Zhang,Yukui Zhang,
来源：第2届大连国际色谱学术报告会、第37届国际高效液相色谱及相关技术会议、第18届全国色谱学术报告会 年份：2011
In clinical proteomics study, serum and plasma are frequently used for the detection of early diagnostic biomarkers.Although mass spectrometry (MS) based proteomic research has played an important rol...
[会议论文] 作者：Yanyan Qu,Huiming Yuan,Lihua Zhang,Zhen Liang,Yukui Zhang,
来源：第2届大连国际色谱学术报告会、第37届国际高效液相色谱及相关技术会议、第18届全国色谱学术报告会 年份：2011
N-glycosylation is one of the most common and complex post-translational modification of proteins, which plays an important role in a variety of biological activities, i.e.receptor-ligand interaction,...
An Integrated Device with Combination of Protein Denaturation, Reduction, Desalting and on-column Di
[会议论文] 作者：Zhongpeng Dai,Lihua Zhang,Zhen Liang,Yukui Zhang,Huiming Yuan,Hao Jiang,
来源：第七届中国蛋白质组学大会暨第三届国际蛋白质组学论坛 年份：2011
...
An Integrated Device with Combination of Protein Denaturation, Reduction, Desalting and on-column Di
[会议论文] 作者：Huiming Yuan,Hao Jiang,Zhongpeng Dai,Lihua Zhang,Zhen Liang,Yukui Zhang,
来源：第七届中国蛋白质组学大会暨第三届国际蛋白质组学论坛 年份：2011
For proteome research,"bottom-up" strategy is one of the most commonly used approaches,by which proteins are in turn denatured,reduced,alkylated,desalted and di...
[会议论文] 作者：Lihua Zhang,Huiming Yuan,Yuan Zhou,Jufeng Ma,Zhen Liang,Yukui Zhang,
来源：The 24th International Symposium on Microscale Bioseparation 年份：2009
...
[期刊论文] 作者：Huiming Yuan,Zhongpeng Dai,Xiaodan Zhang,Baofeng Zhao,Hongwei Chu,Lihua Zhang,Yukui Zhang,
来源：中国科学：化学（英文版） 年份：2021
The bottom-up strategy for proteome analysis typically employs a multistep sample preparation workflow that suffers from being time-consuming and sample loss or...
An efficient proteomic strategy for membrane proteome analysis combining protein prefractionation by
[会议论文] 作者：Oun Zhao,Liangliang Sun,Huiming Yuan,Qi Wu,Zhen Liang,Lihua Zhang,Yukui Zhang,
来源：第2届大连国际色谱学术报告会、第37届国际高效液相色谱及相关技术会议、第18届全国色谱学术报告会 年份：2011
Integral membrane proteins (IMPs) play a critical role in regulating a host of cellular processes, including intercellular communication, vesicle trafficking, ion transport, protein translocation/inte...
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