KNOCKOUT相关论文
Objective::The zebrafish is an excellent model for studying gene function in auditory system development. n Pou4f3 ......
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Reduced prostate branching morphogenesis in stromal fibroblast, but not in epithelial, estrogen rece
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The hemorrhagic disease of grass carp (Ctenopharyngodon idellus) induced by grass carp reovirus (GCRV) leads to huge eco......
Purpose To explore the expression of microRNA in Eaf2 knockout mouse,and the effect of Eaf2 on both human lens epithelia......
Regulation of cPKCγ on phosphorylation status of synapsin-I and their role in cerebral ischemia/hypo
Previously,we have reported that conventional protein kinase C(cPKC)γ was involved in the protective effect of mouse ce......
Deletion of MLCK in endothelial cell has minor effect on lipopolysaccharide induced vascular endothe
Prevalent proposals suggest that myosin light chain kinase (MLCK) plays a critical role in endothelial permeability.To d......
WDR62 was indentified as the second most causative gene of microcephaly.Microcephaly is a neurodevelopmental disorder ch......
Objective The non-coding miRNAs are processed in a step-wise manner from the genomic DNA sequences through pri-miRNAs an......
Objective The strategy to investigate an individual miRNA function in vivo is to delete or to overexpress it in animals.......
Heritable Targeted Inactivation of myostatin b Gene In Yellow Catfish(Tachysurus fulvidraco)using tr
Myostatin(Mstn)is a negative regulator of mammalian muscle growth.Though it has duplicated copies of mstn in fish,little......
Protein Phosphatase 1 Inhibitor-2 Signaling in Synaptic Plasticity and Memory Formation Inhibitor-2:
Protein phosphatase-1(PP1)plays a critical role in synaptic plasticity and memory formation.PP1 activity is required for......
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AIM: To investigate the roles of lymphocytes in the development of dextran sulfate sodium-induced colitis.METHODS: Using......
目的 探讨血管紧张素1a型受体(AT1aR)基因敲除小鼠肾脏局部肾素-血管紧张素系统(RAS)的组分改变对糖尿病肾病(DN)肾小球硬化的影响......
目的 观察瑞舒伐他汀抗血管壁炎症的作用.方法 载脂蛋白E基因敲除小鼠80只和C57BL/6小鼠20只,均为8～9周龄.分为2周药物处理组和6周......
1992年克隆出阿片δ受体后，已从不同种属的动物克隆出了阿片受体的三个亚型μ、δ和k受体。最近法国Kieffer实验室的Matthes等利用......
Flow cytometry,BrdU incorporation,and west blotting were used to investigate whether caveolin-1（Cav-1） is involved in cell......
,Mup-knockout mice generated through CRISPR/Cas9-mediated deletion for use in urinary protein analys
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该文从挂篮荷载计算、施工流程、支座及临时固结施工、挂篮安装及试验、合拢段施工、模板制作安装、钢筋安装、混凝土的浇筑及养生......
目的探讨吡咯并喹啉醌（pyrroloquinoline quinone, PQQ）对Bmi-1基因敲除（Bmi-1 knock out，BKO）小鼠的下颌骨及牙发育障碍的治疗作用及其......
目的利用成簇的规律间隔的短回文重复序列（clustered regularly interspaced short palindromic repeats，CRISPR）／常间回文重复序列丛......
目的：构建红细胞分化相关基因（ EDAG）敲除小鼠并研究其对低剂量辐射损伤的敏感性。方法利用锌指核酸酶技术（ ZFNs）建立EDAG敲除小鼠模型......
目的 探讨基因Abro1在脂多糖(LPS)诱导的急性肺损伤中的作用.方法 Abro1基因敲除型(KO)小鼠和野生型(WT)C57BL/6小鼠各14只,随机各......
A wide range of research topics in different fields of physics can be addressed by study of the self-conjugate N = Z nuc......
KnockOut作为Photoshop外挂滤镜,可以制作一些特效图像,还可以进行精细抠图,本文介绍了KnockOut作为外挂滤镜的安装方法及基本操作......
本文中 ,我们基于Knockout交换结构 ,提出了一种能实现点到多点交换的ATM交换结构 ,并对其性能进行了分析 ,然后通过模拟证明该方......
目的 研究雄激素受体(AR)敲除小鼠睾丸生精功能的变化及曲细精管的p63表达.方法 采用Cre-lox方法筛选出8只AR敲除小鼠,8只AR未被敲......
目的 探讨AP3B型接合蛋白复合体μ亚基基因敲除小鼠(AP3M2KO小鼠)的(癎)性发作特征及机制.方法 建立和繁殖AP3M2KO小鼠,利用远隔摄......
目的 探讨转录因子NF-E2相关因子2(Nrf2)对小鼠脊髓损伤后脊髓内肿瘤坏死因子-α(TNF-α)及基质金属蛋白酶-9(MMP-9)表达的影响.方......
目的 评价牙龈卟啉单胞菌(Porphyromonas gingivalis,Pg)口腔内低剂量、长期、多次接种是否可影响载脂蛋白基因敲除小鼠(apolipopr......
目的 探讨瑞舒伐他汀对载脂蛋白E基因敲除(apoE-/-)小鼠主动脉粥样硬化的影响.方法 取apoE-/-小鼠18只建立动脉粥样硬化模型,C57BL......
Mdm2 and Mdm4 are two key negative regulators of the tumor suppressor p53.Deletion of either Mdm2 or Mdm4 induces p53-de......
目的 利用载脂蛋白E(ApoE)基因敲除小鼠,观察气管滴注纳米二氧化钛(nano-TiO2)颗粒物对脂代谢、动脉粥样硬化症发展的影响及其机制......
目的:评价水通道蛋白4(AQP4)在博莱霉素诱导的小鼠肺纤维化中的作用.方法:采用博莱霉素3 mg/kg气管内注射诱导AQP4基因敲除(AQP4-/......
目的利用CRISPR/Cas9慢病毒系统在人结肠癌细胞(HCT116细胞)中建立稳定及永久性Apak敲除细胞系,检测Apak敲除后细胞部分生物学活性......
功能基因组学的发展促进基因敲除技术的进步,基因敲除技术从载体的构建到细胞筛选再到动物模型都取得长足进步。基因敲除手段种类......
目的构建兔HPRT基因打靶载体。方法在已经筛选到含有兔全长次黄嘌呤一鸟嘌呤磷酸核糖转移酶（Hypoxanthine guanine phosphoribosyl ......
目的应用CRISPR/Cas9技术靶向敲除编码小鼠T、B细胞的Rag2基因及编码NK细胞的IL2rg基因,构建T、B细胞及NK细胞联合免疫缺陷小鼠。......
为了获得高产反式-4-羟脯氨酸的菌株,基于大肠杆菌的代谢网络模型的指导,以大肠杆菌E.coli BL21（DE3）Δput A为出发菌株,通过基因敲......
目的：建立HSF1^-/-，HSF1^＋/＋两种基因型小鼠胚胎成纤维永生化细胞系，为HSF1的功能研究提供实验模型。方法：用脂质体介导的基因转染法将pS......
目的 探讨BAMBI-/-小鼠的繁殖和鉴定方法,筛选出足够数量的BAMBI-/-小鼠,为研究BAMBI基因在肥胖、能量代谢等方面的生理功能提供实......
BACKGROUND Claudin-7,one of the important components of cellular tight junctions,is currently considered to be expressed......
目的：探讨激动β3肾上腺素受体（β3-AR）对载脂蛋白E基因敲除（apoE-/-）小鼠血糖、胰岛素和胰腺血管紧张素Ⅱ受体（ATR）表达水平的影响。方法......
目的研究GRP78／Bip在GJB2基因条件敲除小鼠耳蜗中的表达，探讨内质网应激（endoplasmiereticu-lumstress，ERS）与非综合征型聋的相关性。方......
Dear Editor,Genetic inactivation or gene knockout(KO)has been a powerful method of elucidating gene function in mice.The......
Genetically engineered mouse(GEM)models are commonly used in biomedical research.Generating GEMs involve complex set of ......
