论文部分内容阅读
OBJECTIVE Plasmonic nanostructures act as a type of promising candidate for cancer photothermal therapy.These photothermal agents with good biocompatibility and high photothermal conversion efficiency are highly desirable.In the present study,we synthesized poly(diallyldimethylammonium chloride)(PDDAC)coated porous platinum(Pt)nanoparticles for photothermal therapy.METHODS Biocompatibility and cellular uptake of Pt nanoparticles were studied in human glioblastoma U-87 MG cells.Cell viability was evaluated by ATP assay and calcein AM staining.The photothermal therapeutic effect of the Pt nanoparticles was studied under 808-nm laser irradiation.In addition,the synergistic anti-cancer effect of the Pt nanoparticle-based photothermal therapy and doxorubicinwas investigated.RESULTS The as-prepared Pt nanoparticles exhibited considerable photothermal conversion efficiency under 809 nm and 980 nm laser irradiation.In vitro study indicated that the Pt nanoparticles displayed good biocompatibility and high cellular uptake efficiency.In the presence of the Pt nanoparticles,808-nm laser irradiation at 8.4 W·cm-2for3 min induces significant cytotoxicity,and cell necrosis is involved in the photothermal injury.Furthermore,simultaneousapplication of photothermal therapy synergistically enhances the cytotoxicity of anticancer drug doxorubicin.CONCLUSION Therefore,PDADMAC-coated Pt nanoparticles will have great potential in cancer photothermal therapy.
OBJECTIVE Plasmonic nanostructures act as a type of promising candidate for cancer photothermal therapy. These photothermal agents with good biocompatibility and high photothermal conversion efficiency are highly. In the present study, we synthesized poly (diallyldimethylammonium chloride) (PDDAC) coated porous platinum (Pt ) nanoparticles for photothermal therapy. METHODS Biocompatibility and cellular uptake of Pt nanoparticles were studied in human glioblastoma U-87 MG cells. Cell viability was evaluated by ATP assay and calcein AM staining. The photothermal therapeutic effect of the Pt nanoparticles was studied under 808- nm laser irradiation. In addition, the synergistic anti-cancer effect of the Pt nanoparticle-based photothermal therapy and doxorubicinwas investigated. RESULTS The as-prepared Pt nanopiolate demonstrated substantial photothermal conversion efficiency under 809 nm and 980 nm laser irradiation. that the Pt nanoparticles displayed good biocompatibil ity and high cellular uptake efficiency. The presence of the Pt nanoparticles, 808-nm laser irradiation at 8.4 W · cm -2 for 3 min induces significant cytotoxicity, and cell necrosis is involved in the photothermal injury. Stillrther, simultaneous application of photothermal therapy synergistically enhances the cytotoxicity of anticancer drug doxorubicin. CONCLUSION PDADMAC-coated Pt nanoparticles will have great potential in cancer photothermal therapy.