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目的:探讨海藻氨酸(Kainicacid,KA)致痫后海马神经元谷氨酸转运体(glutamatetransporters,GluTs)功能的变化,并观察牛磺酸(Taurine,Tau)对KA致痫及致痫后海马GluTs功能的影响。方法:40只Wistar大鼠随机分为对照组(Ⅰ组)、KA组(Ⅱ组)、Tau低剂量组(Ⅲ组)、Tau高剂量组(Ⅳ组),每组各10只。Ⅲ组和Ⅳ组提前3d分别腹腔注射低剂量(1g/kg)和高剂量(2g/kg)Tau,然后与Ⅱ组一起腹腔注射KA10mg/kg,诱导癫痫发作,Ⅰ组腹腔注射生理盐水。观察各组的痫性发作情况,并于24h后剥离右侧海马,制备海马突触颗粒,测定其摄取3HL谷氨酸的功能。结果:与对照组相比,KA组点燃后海马突触颗粒GluTs功能降低(P<0.01);与KA组相比,牛磺酸组的致痫潜伏期延长,点燃率、痫性发作分级及死亡率均降低,海马神经元GluTs功能增强,其作用呈剂量依赖性。结论:KA可造成海马神经元GluTs功能降低,Tau抑制KA引起的癫痫发作,其原因可能与部分改善海马神经元GluTs功能有关。
Objective: To investigate the changes of glutamate transporter (GluTs) function in hippocampal neurons induced by kainic acid (KA) and to observe the effect of taurine (Tau) on GluTs Effect of function. Methods: Forty Wistar rats were randomly divided into control group (group Ⅰ), KA group (group Ⅱ), Tau low dose group (group Ⅲ) and Tau high dose group (group Ⅳ). Rats in group Ⅲ and group Ⅳ were injected intraperitoneally with low dose (1g / kg) and high dose (2g / kg) Tau intraperitoneally three days earlier. Group Ⅱ was given intraperitoneal injection of KA 10mg / kg to induce seizure, and group Ⅰ was injected intraperitoneally with saline. The epileptic seizures in each group were observed. The hippocampus of right hippocampus was dissected 24h later, and hippocampal synaptic granules were prepared and the function of uptake of 3H-glutamic acid was determined. Results: Compared with the control group, the function of GluTs in the hippocampal synaptic hippocampus of KA group was decreased after KA administration (P <0.01). Compared with KA group, the latency of epilepticus induced by taurine prolongation, ignition rate, epileptic seizure and death The rates of GluTs in hippocampal neurons were increased, and their effects were dose-dependent. Conclusion: KA can decrease the GluTs function of hippocampal neurons and inhibit the seizures induced by KA by Tau, which may be related to the partial improvement of GluTs function in hippocampal neurons.