论文部分内容阅读
目的 研究 As2 O3引起肿瘤细胞凋亡和对细胞周期的影响。方法 采用 DNA凝胶电泳 ,流式细胞仪分析、RT- PCR和 Western免疫印迹等技术检测细胞凋亡的发生。结果 由 As2 O3诱导的人肺腺癌 GL C- 82、胃腺癌 MGC- 80 3和 SGC- 790 1、食管癌 Ec10 9、宫颈癌 He L a细胞凋亡前 ,细胞阻滞在 G2 +M期 ,上述细胞均表现为对c- myc基因的下行调节 ;但在导致永生化人宫颈上皮 HCE16 /3细胞凋亡之前 ,细胞却被阻滞在 G1期 ,对 c- m yc基因表达无影响。结论 As2 O3引起细胞凋亡与细胞周期阻滞有关 ,在肿瘤细胞和前恶变的 HCE16 /3细胞中阻滞的时期不同 ,这可能与 c- myc基因表达的改变与否有关
Objective To study the effect of As2O3 on tumor cell apoptosis and cell cycle. Methods The apoptosis was detected by DNA gel electrophoresis, flow cytometry, RT-PCR and Western immunoblotting. Results As2O3-induced human lung adenocarcinoma GL C-82, gastric adenocarcinoma MGC-803 and SGC-790 1, esophageal cancer Ec109, cervical cancer He L a cell apoptosis, cell arrest in G2 + M phase These cells all showed down-regulation of the c-myc gene; however, before the immortalization of human cervical epithelial HCE16/3 cells, the cells were arrested in the G1 phase and had no effect on c-myc gene expression. Conclusion As2O3 induces cell apoptosis and cell cycle arrest. The period of arrest is different between tumor cells and premalignant HCE16/3 cells. This may be related to the change of c-myc gene expression.