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本文建立了一个用于检测军团菌的多聚酶链反应方法。扩增参考菌株的染色体ＤＮＡ（Ｌ．ｐｎｅｕｍｏｐｈｉｔａｌ－１４、Ｌ．ｍｉｃｄａｄｅｉ、Ｌ．ｄｕｍｏｆｆｉｉＬｌｏｎｇｂｅａｃｈａｅ、Ｌ．Ｊｏｒｄａｎｉｓ、Ｌ．ｂｏｚｅｍａｎｉｉ），均可检出３７５ｂｐ的１６ＳｒＲＮＡ基因片段。对于已经监定的５株国内分离菌株染色体ＤＮＡ进行扩增，获得了相同的结果，地高辛标记１６ＳｒＲＮＡ基因探针与扩增产物杂交，结果为阳性。而扩增１４株非军团菌均为阴性。采用煮沸法制备细菌染色体ＤＮＡ，ＰＣＲ法检测环境水军团菌敏感性为２８０ｃｆｕ／ｍｌ水，检查临床标本军团菌为５６０ｃｆｕ／ｍｌ支气管灌洗液。上述结果表明该法敏感、快速、简便，具有较好的特异性。
This article established a method for the detection of Legionella by polymerase chain reaction. Amplification of reference strains of chromosomal DNA (L.pneumophital-14, L.micadensis, L.dumoffiiLlongbeachae, L.Jordanis, L.bozemanii), can be detected 375bp16SrRNA gene fragment. The same result was obtained by amplifying the chromosomal DNA of the 5 isolates already identified. The 16SrRNA gene probe labeled with digoxigenin was hybridized with the amplification product, and the result was positive. The amplification of 14 non-Legionella were negative. The bacterial chromosomal DNA was prepared by boiling method. The sensitivity of Legionella pneumophila was 280cfu / ml water by PCR method and the bronchial lavage fluid of 560cfu / ml was detected by clinical samples Legionella. The above results show that the method is sensitive, fast, simple and has good specificity.