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本法主要采用EagLes液(Minima Essentia/Medium简称MEM)千倍稀释NDV-F病毒接种鸡胚,结果表明,用MEM液千倍稀释NDV-F接种鸡胚的血凝滴度比用NaCl液百倍稀释NDV-F的鸡胚的血凝滴度高,而其接种后的鸡胚成活率高,收集的NDV-F液量多。
In this method, the chicken embryos were inoculated with a thousand-fold dilution of NDV-F virus by using EagLes solution (MEM). The results showed that the hemagglutination titer of NDV-F inoculated with the thousandfold dilution of MEM solution was 100 times higher than that of NaCl solution Chicken chick embryos diluted with NDV-F had high titer of hemagglutination while their inoculated chick embryos had high survival rate and collected more NDV-F fluid.