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目的:观察琼玉膏对顺氨氯铂抑制肺腺癌细胞株GLC-82增殖的影响。方法:使用血清药理学分别观察空白对照组(生理盐水)、化疗组(顺氨氯铂)及联合组(顺氨氯铂加琼玉膏)细胞形态学的变化;使用活细胞计数方法记录各组癌细胞6日的生长情况并绘制其生长曲线;使用流式细胞等方法分析各组癌细胞株GLC-82的凋亡情况。结果:对照组癌细胞生长良好,化疗组癌细胞数基本不增长,联合组活癌细胞数则下降。流式细胞仪的分析结果表明,琼玉膏可明显加强化疗药物顺氨氯铂(DDP)诱发癌细胞的凋亡。结论:琼玉膏有加强化疗抑制癌细胞分裂及诱发癌细胞凋亡的作用。
Objective: To observe the effect of Qiongyu ointment on the proliferation of lung adenocarcinoma cell line GLC-82 induced by cisplatin. Methods: Using serum pharmacology to observe the changes of cell morphology in control group (normal saline), chemotherapy group (cisplatin) and combination group (cisplatin plus Qiongyu cream); The growth of the cancer cells on the 6th day was plotted and their growth curves were plotted. Flow cytometry was used to analyze the apoptosis of GLC-82 cells. Results: The cancer cells in the control group grew well, the number of cancer cells in the chemotherapy group did not increase, and the number of cancer cells in the combined group decreased. Flow cytometry analysis showed that Qiong Yu Ointment can significantly enhance the apoptosis of cancer cells induced by chemotherapeutic drug cisplatin (DDP). Conclusion: Qiongyu ointment can enhance the effect of chemotherapy on inhibiting the division of cancer cells and inducing the apoptosis of cancer cells.