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目的探讨血清p53抗体检测在肺癌早期诊断、疗效判定及判断预后中的临床意义。方法应用ELISA法检测华北煤炭医学院附属开滦医院2000年1月至2003年4月间收治的76例肺癌患者血清p53抗体滴度,经酶联免疫检测仪测定吸光值E450,计算p53抗体指数,进行统计学分析。结果76例中有37例p53抗体阳性,阳性率48.68%;在早期(Ⅰ期)肺癌中,p53抗体阳性率为65.21%;明显高于癌胚抗原(CEA)的阳性率(P<0.05);血清p53抗体与CEA联合检测对肺癌的检出率明显高于单独血清CEA检测对肺癌检出率(P<0.01)。手术治疗、化疗及未接受治疗的3组肺癌患者的p53抗体分别呈现动态降低或升高变化,显示p53抗体与肿瘤负荷、病程及病情恶化程度密切相关(P<0.01及P<0.05)。结论血清p53抗体检测有助于肺癌的诊断及早期诊断;其动态变化规律有助于监测病情、判断疗效及判断预后。
Objective To investigate the clinical significance of serum p53 antibody detection in early diagnosis of lung cancer, determination of therapeutic effect and judgment of prognosis. Methods Serum p53 antibody titers of 76 patients with lung cancer admitted to Kailuan Hospital, North China Coal Medical College from January 2000 to April 2003 were detected by enzyme-linked immunosorbent assay (ELISA), and the absorbance (E450) was measured by enzyme-linked immunosorbent assay , For statistical analysis. Results Of the 76 cases, 37 cases were positive for p53 antibody and the positive rate was 48.68%. The positive rate of p53 antibody was 65.21% in early stage (stage Ⅰ) lung cancer, which was significantly higher than that of CEA (P0.05) The detection rate of serum p53 antibody and CEA in lung cancer was significantly higher than that of serum CEA alone in detection of lung cancer (P <0.01). The p53 antibodies in the three groups of lung cancer patients undergoing surgery, chemotherapy and non-treatment showed dynamic decrease or increase, respectively. The results showed that the p53 antibody was closely related to tumor burden, duration of disease and the severity of the disease (P <0.01 and P <0.05). Conclusion The detection of serum p53 antibody is helpful for the diagnosis and early diagnosis of lung cancer. The regularity of its dynamic changes helps to monitor the condition, judge the curative effect and judge the prognosis.