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目的:观察小儿止咳颗粒对急性肺损伤小鼠晚期炎症介质高迁移率族蛋白B-1(high mobility group box-1 protein,HMGB-1)含量的影响。方法:将ICR小鼠随机分成6组,分别测定空白组和脂多糖(LPS)尾iv致小鼠急性肺损伤后24,36,48,72,96 h血清中HMGB-1的含量,确定其含量最高的时间点。将ICR小鼠随机分为空白对照组、模型组、小儿止咳颗粒高、中、低剂量组,醋酸地塞米松组;除空白组外,各组尾iv LPS造成急性肺损伤模型,给药组分别ig给予小儿止咳颗粒5.4,2.7,1.35g·kg-1及醋酸地塞米松0.5 mg·kg-1,空白组ig给予等量生理盐水,在iv LPS 72 h测定各组小鼠血清中HMGB-1的含量。结果:iv LPS后72 h,小鼠血清中HMGB-1的含量最高,在该时间点,与模型组相比小儿止咳颗粒高、中、低剂量均能够抑制HMGB-1含量的增高(P<0.05)。结论:小儿止咳颗粒对晚期炎症介质HMGB-1含量的增高具有一定的抑制作用,这可能是其发挥治疗小儿上呼吸道感染后咳嗽作用的机制之一。
Objective: To observe the effect of Xiaoer Zhike Granule on the content of high mobility group box-1 protein (HMGB1) in the late inflammatory mediators of acute lung injury in mice. Methods: The ICR mice were randomly divided into 6 groups, and the levels of HMGB-1 in the serum were determined respectively at 24, 36, 48, 72 and 96 h after acute lung injury induced by lipopolysaccharide (LPS) The highest point of time. The ICR mice were randomly divided into blank control group, model group, high and low dose Zhiganzi Granule group and dexamethasone acetate group. Except for the blank group, iv LPS induced acute lung injury in each group, Respectively, ig given cough particles in children 5.4,2.7,1.35g · kg-1 and dexamethasone acetate 0.5 mg · kg-1, the blank group ig given the same amount of saline at 72 h iv LPS determination of serum HMGB -1 content. Results: At 72 h after LPS, the serum levels of HMGB-1 in mice were the highest. Compared with the model group, Xiaoli Zhike Granule could inhibit the increase of HMGB-1 content at the time point (P < 0.05). Conclusion: Xiaozhi Zhikue Granule can inhibit the increase of HMGB-1 in late inflammatory mediators, which may be one of the mechanisms that play a role in the treatment of cough after upper respiratory tract infection in children.