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目的 :对比研究丙泊酚和氯胺酮对心肌细胞的毒性作用。方法 :将经原代培养成活 4d后的大鼠心肌细胞分为 4组 ,分别为低浓度 (PL ,3× 10 -5mol/L)与高浓度 (PR ,3× 10 -4 mol/L)丙泊酚组和低浓度 (KL ,1× 10 -5mol/L)与高浓度 (KH ,1× 10 -4 mol/L)氯胺酮组。各组均于实验开始后 8h终止反应 ,评定心肌细胞搏动功能 ,观察细胞形态学改变 ,测定心肌细胞酶及电解质变化。结果 :组间比较 ,KL组心肌细胞搏动频率加快 (P <0 .0 5 ) ;PR和KH组搏动频率明显减慢 (P <0 .0 5 ) ,细胞形态学亦有相应变化 ;LDH ,AST与CK释放量增加 ,ALP活性下降 (P <0 .0 5 ) ;而PL和KL组的上述指标无明显变化 ;各组间电解质变化未见显著差异。结论 :PR和KH组均有直接的心肌抑制作用。而PL和KL组并无心肌细胞毒性作用 ,且KL组表现出正性变时性和变力性作用。
Objective: To compare the toxic effects of propofol and ketamine on cardiomyocytes. Methods: The primary cultured cardiomyocytes were divided into 4 groups: low concentration (PL, 3 × 10 -5 mol / L) and high concentration (PR, 3 × 10 -4 mol / L) Propofol group and low concentrations (KL, 1 × 10 -5 mol / L) and high concentration (KH, 1 × 10 -4 mol / L) ketamine group. Each group was terminated at 8h after the start of the experiment, the cardiomyocyte cardiomyocyte function was assessed, morphological changes of the cells were observed, and enzyme and electrolyte changes of cardiomyocytes were measured. Results: Compared with the control group, the pulsation frequency of cardiomyocytes in KL group was faster (P <0.05). The pulsatile frequency of KLK group was significantly lower in PR and KH groups (P <0.05) AST and CK release increased, ALP activity decreased (P <0. 05); while the PL and KL group no significant changes in the above indicators; electrolyte changes between groups showed no significant difference. Conclusion: PR and KH groups have direct myocardial inhibition. However, no cytotoxicity was observed in PL and KL groups, and KL group showed positive chronotropic and inotropic effects.