以“津春4号”黄瓜为试材,通过测定黄瓜叶片叶绿素荧光快速诱导动力学曲线和对820nm光的吸收曲线,结合叶绿素荧光淬灭分析,研究低温光胁迫(4℃,200μmol·m-2·s-1)6h后,黄瓜叶片在常温(25℃)不同光强(0、15、200μmol·m-2·s-1)下PSⅠ和PSⅡ活性的恢复,以及恢复过程中PSⅠ与PSⅡ的相互作用.结果表明:低温光胁迫6h后,PSⅠ和PSⅡ发生不同程度的光抑制.在常温恢复阶段,PSⅡ活性快速恢复且对光强不敏感;PSⅠ活性在弱光下(15μmol·m-2·s-1)快速恢复,在较强光(200μmol·m-2·s-1)下恢复较慢.在低温光抑制恢复过程中,常温下PSⅡ活性恢复较快可能导致PSⅡ向PSⅠ的线性电子传递过快,进而抑制PSⅠ的活性恢复.因此,在进行黄瓜抗冷性育种时,不应该仅追求较高的PSⅡ抗性和较快的PSⅡ恢复速度,还应该注意两个光系统活性的协调.在生产中,应当在低温逆境发生及其之后较长一段时间内采取措施降低叶表面光照强度,以利于对植株光合机构的保护和光合活性的恢复. The cucumber with “Jinchun No.4” was used as test material to measure the fast kinetics curve of the chlorophyll fluorescence and the absorption curve to the light of 820nm, and the chlorophyll fluorescence quenching analysis was used to study the effects of low temperature light stress (4 ℃, 200μmol · m-2 · s-1) for 6 h, the recovery of PSⅠ and PSⅡ activity of cucumber leaves under different light intensities (0, 15, 200μmol · m-2 · s-1) And PSⅡ.Results showed that PS Ⅰ and PSⅡhad different degrees of photoinhibition at low temperature and light stress for 6h.PSⅡ activity recovered quickly and was insensitive to light intensity at normal temperature recovery stage.The activity of PSⅠ in low light (15μmol · m-2 · s-1), and recover more slowly under higher light (200μmol · m-2 · s-1) .It is suggested that the recovery of PSⅡ activity at room temperature Therefore, in the chilling tolerance breeding of cucumber, we should not only pursue higher PSⅡresistance and faster PSⅡrecovery rate, but also pay attention to the two light Coordination of system activity In production, it should be taken at a later time after the advent of cold stress Measures to reduce the light intensity of the leaf surface, in order to facilitate the protection of photosynthetic apparatus and photosynthetic plant activity recovery.