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目的观察原癌基因c-myc在糖尿病大鼠心肌肥大中的表达及胰岛素对其干预作用。方法腹腔注射链脲菌素60 mg.kg-1诱导大鼠糖尿病模型,1、3及7天时分别测定其血糖及体重情况。第3天时提取左心室总RNA,采用PT-PCR法测定原癌基因c-myc的表达。结果正常组大鼠的平均血糖水平为5.55±1.23 mmol.L-1,糖尿病组大鼠在腹腔注射链脲菌素后1、3、7天其血糖水平分别为21.92±6.94 mmol.L-1、26.42±1.82 mmol.L-1、28.98±7.27 mmol.L-1,胰岛素预处理组大鼠在腹腔注射链脲菌素后1、3、7天其血糖水平分别为16.59±1.13 mmol.L-1、18.34±1.82 mmol.L-1、17.77±1.22 mmol.L-1。3天时糖尿病组大鼠原癌基因c-myc的表达量是对照组的4倍,胰岛素预处理可以显著抑制c-myc原癌基因的表达。结论糖尿病大鼠的心肌肥大与c-myc原癌基因的过多表达有关,胰岛素预处理可抑制c-myc原癌基因的表达。
Objective To observe the expression of proto-oncogene c-myc in cardiac hypertrophy of diabetic rats and the effect of insulin on it. Methods Diabetic rats were induced by intraperitoneal injection of streptozotocin at a dose of 60 mg · kg-1, and their blood glucose and body weight were measured at 1, 3 and 7 days respectively. Left ventricular total RNA was extracted on day 3 and the expression of proto-oncogene c-myc was measured by PT-PCR. Results The average blood glucose level of rats in normal group was 5.55 ± 1.23 mmol.L-1. The blood glucose levels of diabetic rats were 21.92 ± 6.94 mmol.L-1 on the 1st, 3rd and 7th days after intraperitoneal injection of streptozotocin , 26.42 ± 1.82 mmol.L-1, 28.98 ± 7.27 mmol.L-1 respectively. The blood glucose levels of rats in the insulin preconditioning group on the 1st, 7th and 7th days after intraperitoneal injection of streptozotocin were 16.59 ± 1.13 mmol.L -1, 18.34 ± 1.82 mmol.L-1, 17.77 ± 1.22 mmol.L-1.3 days, the expression of c-myc in the diabetic rats was four times that of the control group, insulin pretreatment could significantly inhibit the c -myc proto-oncogene expression. Conclusion The cardiac hypertrophy in diabetic rats is related to the overexpression of c-myc oncogene. Pretreatment with insulin can inhibit the expression of c-myc oncogene.