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目的 了解前列腺素 E1 (PGE1 )对日本血吸虫病肝纤维化形成的影响。方法 采用免疫组化 SABC技术 ,并用计算机彩色病理图文分析系统 ,检测分析感染日本血吸虫大鼠经 PGE1 治疗后肝内 、 型胶原及转化生长因子 β1 (TGF- β1 )的含量变化。结果 PGE1 治疗组肝内 、 型胶原及转化生长因子 β1 (TGF- β1 )的含量分别低于感染对照组 (P<0 .0 5 ) ;PGE1 治疗组及感染对照组肝内 、 型胶原的含量均与 TGF- β1 呈显著正相关 (r=0 .96 36 ,P<0 .0 5 ;r=0 .86 6 7,P<0 .0 5 ;r=0 .96 36 ,P<0 .0 5 ;r=0 .92 72 ,P<0 .0 5 )。结论 PGE1 对日本血吸虫病肝纤维化的形成有抑制作用 ,其机理可能与 PGE1 抑制 TGF- β1 的表达有关。
Objective To investigate the effect of prostaglandin E1 (PGE1) on hepatic fibrosis in Schistosoma japonicum. Methods Immunohistochemical SABC technique was used to detect the changes of hepatic collagen type Ⅰ and transforming growth factor β1 (TGF-β1) content in the infected Schistosoma japonicum after PGE1 treatment by computerized color chart analysis system. Results The content of intrahepatic collagen, transforming growth factor-β1 (TGF-β1) in PGE1-treated group was lower than that in infected control group (P <0.05), PGE1-treated group and infected control group There was a significant positive correlation with TGF-β1 (r = 0.9636, P <0.05; r = 0.8667, P <0.05; r = 0.9636, P <0. 0 5; r = 0.92 72, P <0.05). Conclusion PGE1 can inhibit the formation of hepatic fibrosis in schistosomiasis japonica, which may be related to the inhibition of PGE1 expression by TGF-β1.