目的评定紫外分光光度法测定保健食品中茶多酚含量的不确定度。方法 JJF 1059.1-2012依据《测量不确定度评定与表示》,分析实验过程中茶多酚含量测定的不确定度来源;通过建立数学模型以量化不确定度分量,计算合成不确定度及扩展不确定度。结果保健食品中茶多酚的含量为1.08%,其扩展不确定度为0.15%(k=2)。结论紫外分光光度法测定保健食品中茶多酚含量的不确定度主要来源于标准曲线拟合、回收率及紫外分光光度计等3方面,其贡献率分别为54.8%、20.8%和12.5%。 Objective To assess the uncertainty of the determination of tea polyphenols in health food by ultraviolet spectrophotometry. Method JJF 1059.1-2012 According to “Evaluation and Expression of Uncertainty in Measurement”, the sources of uncertainty in the determination of tea polyphenols during the experiment were analyzed. The mathematical uncertainty was calculated by establishing a mathematical model to calculate the uncertainty of synthesis and expansion Determine the degree. Results The content of tea polyphenols in health food was 1.08% with an uncertainty of expansion of 0.15% (k = 2). Conclusion The uncertainty of UV detection of tea polyphenols in health food mainly comes from three aspects: standard curve fitting, recovery and UV spectrophotometer. The contribution rates are 54.8%, 20.8% and 12.5% ​​respectively.