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目的探讨链脲佐菌素(STZ)诱导的糖尿病小鼠肾组织中蛋氨酸亚砜还原酶B1(MsrB1)的表达变化及其与氧化应激的关系。方法将10周龄雄性C57BL/6小鼠随机分为4组:正常对照组(NC组)、单侧肾切除组(UX组)、STZ组和单侧肾切除加STZ组(STZ-UX组)。模型制备后第8周末,免疫组化法检测MsrB1在肾组织中的表达和分布;实时定量PCR、Western blotting检测MsrB1 mRNA及蛋白水平的变化;通过丙二醛(MDA)、蛋白羰基(PC)和总巯基(TSH)测试盒检测氧化损伤情况。结果 MsrB1表达于小鼠肾小管上皮细胞的细胞核和细胞质。与NC组相比,STZ组和STZ-UX组MsrB1表达量和TSH含量减少(P<0.05),而MDA、PC含量增加(P<0.05),且STZ-UX组比STZ组变化更明显;UX组没有明显改变(P>0.05)。相关性分析结果显示,STZ组和STZ-UX组MsrB1蛋白表达与MDA和PC呈负相关(P<0.05),与TSH含量呈正相关(P<0.05)。结论在STZ诱导的糖尿病小鼠肾组织中,MsrB1表达明显减少,这种变化可能参与了糖尿病肾病(DN)氧化应激的发生。
Objective To investigate the expression of methionine sulfoxide reductase B1 (MsrB1) in renal tissue of streptozotocin (STZ) -induced diabetic mice and its relationship with oxidative stress. Methods 10-week-old male C57BL / 6 mice were randomly divided into 4 groups: normal control group (NC group), unilateral nephrectomy group (UX group), STZ group and unilateral nephrectomy plus STZ group ). At the end of the 8th week after the model was prepared, the expression and distribution of MsrB1 in renal tissues were detected by immunohistochemistry. The mRNA and protein levels of MsrB1 were detected by real-time PCR and Western blotting. The expression of MsrB1 mRNA and protein was detected by malondialdehyde (MDA) And total mercapto (TSH) test box to detect oxidative damage. Results MsrB1 was expressed in the nucleus and cytoplasm of mouse renal tubular epithelial cells. Compared with NC group, the expression of MsrB1 and the content of TSH in STZ group and STZ-UX group were decreased (P <0.05), while the contents of MDA and PC were increased (P <0.05), and STZ-UX group was more obvious than STZ group. UX group did not change significantly (P> 0.05). Correlation analysis showed that MsrB1 protein expression was negatively correlated with MDA and PC in STZ and STZ-UX groups (P <0.05), and positively correlated with TSH content (P <0.05). Conclusion The expression of MsrB1 in the kidney of STZ-induced diabetic mice is significantly decreased, which may be involved in the oxidative stress of diabetic nephropathy (DN).