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OBJECTIVE This research was to induce dendritic cells (DCs)from mice embryonic stem cells and bone marrow mononuclearcells in vitro, and then compare the biologic characteristics of them.METHODS Embryonic stem cells (ESCs) suspending culturedin petri dishes were induced to generate embryonic bodies (EBs).Fourteen-day well-developed EBs were transferred to histologicalculture with the same medium and supplemented 25 ng/ml GM-CSF and 25 ng/ml IL-3. In the next 2 weeks, there were numerousimmature DCs outgrown. Meantime, mononuclear cells isolatedfrom mice bone marrow were induced to derive dendritic cells bysupplementing 25 ng/ml GM-CSF and 25 ng/ml IL-4, and then themorphology, phenotype and function of both dendritic cells fromdifferent origins were examined.RESULTS Growing mature through exposure tolipopolysaccharide (LPS), both ESC-DCs and BM-DCs exhibiteddramatic veils of cytoplasm and extensive dendrites on theirsurfaces, highly expressed CD11c, MHC-Ⅱ and CD86 with strongcapacity to stimulate primary T cell responses in mixed leukocytereaction (MLR) .CONCLUSION ESC-DC has the same biologic characteristics asBM-DC, and it provides a new, reliable source for the functionalresearch of DC and next produce corresponding anti-tumorvaccine.
OBJECTIVE This research was to induce dendritic cells (DCs) from mice embryonic stem cells and bone marrow mononuclear cells in vitro, and then compare the biologic characteristics of them. METHODS Embryonic stem cells (ESCs) suspending culturedin petri dishes were induced to generate embryonic bodies EBs). Fourteen-day well-developed EBs were transferred to histological culture with the same medium and supplemented with 25 ng / ml GM-CSF and 25 ng / ml IL-3. mononuclear cells isolatedfrom mice bone marrow were induced to derive dendritic cells bysupplementing 25 ng / ml GM-CSF and 25 ng / ml IL-4, and then theyorphology, phenotype and function of both dendritic cells from different origins were examined .RESULTS Growing mature through exposure tolipopolysaccharide (LPS), both ESC-DCs and BM-DCs exhibited differential veils of cytoplasm and extensive dendrites on theirsurfaces, highly expressed CD11c, MHC-II and CD86 with strong capacity to stimulate primary T cell responses in mixed leukocyte reactions (MLR) .CONCLUSION ESC-DC has the same biologic characteristics as BM-DC, and it provides a new, reliable source for the functional research of DC and next produce corresponding anti-tumorvaccine.