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目的:探讨Bax和PHF20蛋白在喉鳞状细胞癌(LSCC)中的表达情况及临床意义。方法:应用免疫组织化学SP法检测LSCC组织及癌旁正常黏膜组织中Bax与PHF20的表达情况,分析Bax和PHF20的表达与LSCC患者的临床分型、病理类型、组织学分级及淋巴结转移等临床病理参数之间的关系。结果:1与癌旁切缘正常喉黏膜组比较,LSCC组中Bax和PHF20的表达量均明显降低,差异均有统计学意义(P<0.01)。2在临床分型中,LSCC声门上型、声门型、声门下型3组中Bax和PHF20蛋白表达量及表达阳性率差异均无统计学意义(P>0.05)。在组织学分化分组及病理学分型中,Bax和PHF20蛋白表达量及阳性表达率在低分化的LSCC组织中与高、中分化的LSCC组织相比,均显著降低(分别为P<0.01与P<0.05)。在T分期分组中,Bax和PHF20蛋白表达量及阳性率在T1+T2组均显著高于T3+T4组(均P<0.01)。在有无淋巴结转移分组中,无淋巴结转移的LSCC组织中Bax和PHF20的表达量及阳性率均明显高于发生淋巴结转移者(均P<0.01)。结论:Bax和PHF20的缺失可能与LSCC的发生及进展有关。进一步研究Bax和PHF20对LSCC细胞生物学行为的影响以及可能的分子调控机制,为明确LSCC发生发展提供了新的思路。
Objective: To investigate the expression of Bax and PHF20 protein in laryngeal squamous cell carcinoma (LSCC) and its clinical significance. Methods: The expressions of Bax and PHF20 in LSCC tissues and adjacent normal mucosa tissues were detected by immunohistochemical SP method. The expressions of Bax and PHF20 were compared with those of LSCC patients in clinical classification, pathological type, histological grade and lymph node metastasis Relationship between pathological parameters. Results: 1 Compared with normal laryngeal mucosa, the expression of Bax and PHF20 in LSCC group were significantly decreased (P <0.01). 2 In the clinical classification, there was no significant difference in the expression of Bax and PHF20 protein in the supraglottic, glottis and subglottic LSCC (P> 0.05). In histological differentiation group and pathological classification, the expression and the positive rate of Bax and PHF20 protein in LSCC tissues with low differentiation were significantly lower than those in LSCC tissues with high differentiation (P <0.01 and P <0.05). In T stage group, Bax and PHF20 protein expression and positive rate in T1 + T2 group were significantly higher than T3 + T4 group (P <0.01). In the group with or without lymph node metastasis, the expression and positive rate of Bax and PHF20 in LSCC without lymph node metastasis were significantly higher than those with lymph node metastasis (all P <0.01). Conclusion: The deletion of Bax and PHF20 may be related to the occurrence and progression of LSCC. Further study of the influence of Bax and PHF20 on the biological behavior of LSCC cells and the possible molecular regulation mechanism provide a new way to clarify the occurrence and development of LSCC.