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本实验应用电子顺磁共振技术测定家兔 SMAO 休克时小肠组织在缺血、再灌流前后自由基的改变,同时检测动脉血丙二醛(MDA)含量的变化。18只家兔随机均分为三组:对照组、东莨菪碱治疗组(S组)、过氧化氢酶治疗组(CAT 组)。结果显示:肠系膜上动脉夹闭1小时小肠组织自由基浓度明显增加(P<0.05),再灌流30分钟后自由基浓度进一升高(P<0.01)。S 组和 CAT 组再灌流30分钟时,小肠组织中自由基浓度明显低于对照组。MDA 检测结果类似。本研究结果证明:SMAO 休克小肠组织有大量自由基产生,东莨菪碱和 CAT 可能通过减少自由基的产生或清除自由基而起到缺血、再灌流小肠组织免受损伤的作用。
In this experiment, electron paramagnetic resonance technique was used to determine the change of free radicals in the small intestine tissue before and after ischemia and reperfusion in rabbits with SMAO shock. At the same time, the content of malondialdehyde (MDA) in arterial blood was detected. Eighteen rabbits were randomly divided into three groups: control group, scopolamine treatment group (S group), and catalase treatment group (CAT group). The results showed that the concentration of free radicals in the small intestine of the superior mesenteric artery was significantly increased (P<0.05), and the concentration of free radicals increased further after 30 minutes of reperfusion (P<0.01). After 30 minutes of reperfusion in S group and CAT group, the concentration of free radicals in the small intestine tissue was significantly lower than that in the control group. The MDA test results are similar. The results of this study demonstrate that there are a large number of free radicals in the small intestine tissue of SMAO shock. Scopolamine and CAT may play a role in ischemic injury and reperfusion of small intestine tissue by reducing free radical production or scavenging free radicals.