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目的:研究拳参正丁醇提取物(PBNA)的水溶性成份PBNA-413对脑缺血再灌注损伤的保护作用,并初步探讨其保护作用与炎症反应之间的关系。方法:大鼠大脑中动脉栓塞制作脑缺血再灌注损伤模型,缺血后10分钟,于舌下静脉给不同剂量的PBNA-413。再灌注24h后,断头取脑,TTC染色,测定脑梗死体积;取前脑称湿重,烘干后称干重,计算脑组织含水率;取血2ml,离心后,分离血清,另一组大鼠取大脑组织制成10%的匀浆,放免法测血清及脑组织IL-6、IL-1β及TNFα含量。结果:与模型组相比,PBNA-413治疗组脑梗死体积减小,脑组织含水率降低、IL-1β含量升高、TNFα含量降低,血清IL-6含量降低、IL-1β升高,血清TNFα及脑组织IL-6含量无明显变化。结论:PBNA-413对脑缺血再灌注损伤具有一定的保护作用,其保护机制与降低炎性细胞因子IL-6、IL-1β及TNFα的表达有关。
Objective: To investigate the protective effect of PBNA-413, a water-soluble component of n-butanol extract (PBNA) of Quanshen, on cerebral ischemia-reperfusion injury, and to explore the relationship between its protective effect and inflammatory response. Methods: Cerebral ischemia-reperfusion injury model was established by middle cerebral artery occlusion in rats. Ten minutes after ischemia, different doses of PBNA-413 were given in the sublingual vein. After 24 hours of reperfusion, the brain was removed and the brain was removed. TTC staining was performed to measure the volume of cerebral infarction. The forebrain was weighed and the dry weight was weighed. After drying, the brain tissue water content was calculated. After taking 2 ml of blood, the serum was separated after centrifugation. The rat brain tissue was made into 10% homogenate, serum and brain tissue IL-6, IL-1β and TNFα levels were measured by radioimmunoassay. Results: Compared with the model group, PBNA-413 treatment group’s cerebral infarction volume decreased, brain tissue water content decreased, IL-1β content increased, TNFα content decreased, serum IL-6 content decreased, IL-1β increased, serum TNFα and brain tissue IL-6 content did not change significantly. Conclusion: PBNA-413 has a certain protective effect on cerebral ischemia-reperfusion injury, and its protective mechanism is related to the decrease of inflammatory cytokines IL-6, IL-1β and TNFα expression.