亲水作用色谱(HILIC)作为一种分离强极性化合物的液相色谱模式,近年来在药品、食品分析和代谢组学、蛋白质组学研究等领域受到广泛关注,尤其在天然产物极性成分中的研究应用明显增多。本文基于近年来国内外相关文献,对HILIC的固定相、流动相、分离机理及其在天然产物分离分析中的应用进行了综述和展望,并评述了其不足。 Hydrophilic interaction chromatography (HILIC), as a liquid chromatographic mode for the separation of highly polar compounds, has attracted much attention in recent years in the fields of pharmaceuticals, food analysis and metabolomics, proteomics and other fields, especially in polar components of natural products In the research and application of significant increase. Based on the relevant literature at home and abroad in recent years, this review summarizes and prospects HILIC’s stationary phase, mobile phase, separation mechanism and its application in the separation and analysis of natural products, and reviews its shortcomings.