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Ｋｎｖ－Ⅰ是一种新近发现的抗癌基因，目前对其在白血病发病中的作用研究甚少。利用逆转录多聚酶链反应扩增Ｋｒｅｖ－ⅠｃＤＮＡ片段，然后重组入质粒中而制备出Ｋｒｅｖ－ⅠｃＤＮＡ探针，使用该探针进行Ｎｏｒｔｈｅｒｎ杂交检测Ｋｎｖ－ⅠｍＲＮＡ表达水平，与正常骨髓细胞相比较，发现白血病细胞系ＨＬ－６０、Ｕ－９３７中Ｋｒｅｖ－ⅠｍＲＮＡ表达水平明显降低；１４例急性髓系白血病中，１例表达与正常相当，２例轻度降低，９例明显降低，２例无Ｋｒｅｖ－ⅠｍＲＮＡ表达。说明Ｋｒｅｖ－Ⅰ表达水平的降低在白血病的发生中可能起着重要的作用。
Knv-I is a recently discovered anti-oncogene, and its role in the pathogenesis of leukemia is currently limited. The Krev-I cDNA fragment was amplified by reverse transcription polymerase chain reaction and then recombined into a plasmid to prepare a Krev-I cDNA probe. The probe was used for Northern hybridization to detect the expression level of Knv-I mRNA, and compared with normal bone marrow cells, leukemia was found. The expression level of Krev-I mRNA in the cell lines HL-60 and U-937 was significantly reduced. In 14 cases of acute myeloid leukemia, 1 case was equivalent to normal, 2 cases were mildly decreased, 9 cases were significantly lower, and 2 cases had no Krev-I mRNA. expression. This indicates that the decrease of Krev-I expression level may play an important role in the occurrence of leukemia.