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背景:交感神经系统影响骨代谢的具体作用和机制不明确。目的:探讨普萘洛尔对去卵巢大鼠骨密度及对血清白细胞介素6(IL-6)水平的影响,研究β受体阻滞剂对骨质疏松的防治作用及机制。设计:随机对照的实验研究。地点、材料和干预:本实验在中国中医研究院基础所完成。将50只健康雌性未交配6个月龄SD大鼠随机分为5组:①假手术组。②去卵巢(OVX)后雌激素治疗(O+E)组。③OVX后普萘洛尔治疗(O+P)组。④OVX后雌激素+普萘洛尔治疗(O+E+P)组。⑤OVX后安慰剂(OVX)组。OVX后1周开始经灌胃给予普萘洛尔、皮下注射17β-雌二醇,共12周。动物处死时留取第3～5腰椎、血清标本,进行骨密度分析及血清IL-6水平测定。主要观察指标:各组大鼠椎骨骨密度及血清瘦素水平。结果:O+P,O+P+E,O+E组的骨密度较OVX组明显增高(P<0.05),血清IL-6水平OVX组犤(336.6±18.1)ng/L犦较假手术组犤(208.8±17.2)ng/L犦明显增高(t=16.982,P<0.01),O+E组犤(252.7±16.3)ng/L犦,O+P组犤(274.4±20.0)ng/L犦,O+P+E组犤(222.9±16.5)ng/L犦均明显低于OVX组犤(336.6±18.1)ng/L犦(t=11.160,7.783,14.971,P<0.01)。结论:普萘洛尔能使卵巢切除术后骨质疏松大鼠的骨密度改善,能有效地预防OVI大鼠的骨丢失,同时降低血清水平。
Background: The specific role and mechanism of sympathetic nervous system affecting bone metabolism is not clear. Objective: To investigate the effects of propranolol on bone mineral density (BMD) and interleukin-6 (IL-6) in ovariectomized rats and the preventive and therapeutic effects of β-blocker on osteoporosis. Design: Randomized controlled experimental study. Location, Materials and Interventions: This experiment was completed on the basis of China Academy of Traditional Chinese Medicine. Fifty healthy female un-mating 6-month-old SD rats were randomly divided into 5 groups: ① sham operation group. ② ovariectomized (OVX) estrogen treatment (O + E) group. ③ propranolol after OVX treatment (O + P) group. ④ OVX estrogen + propranolol treatment (O + E + P) group. ⑤OVX post-placebo (OVX) group. Propranolol was given by gavage one week after OVX and 17β-estradiol was injected subcutaneously for 12 weeks. Animals were sacrificed at the 3rd to 5th lumbar spine and serum samples were collected for bone mineral density analysis and determination of serum IL-6 levels. MAIN OUTCOME MEASURES: Vertebral bone mineral density and serum leptin level in each group. Results: The BMD of O + P, O + P + E and O + E groups was significantly higher than that of OVX group (P <0.05), and the level of IL-6 in OVX group was (336.6 ± 18.1) ng / L In the O + E group (252.7 ± 16.3) ng / L 犦 and O + P group 犤 (274.4 ± 20.0) ng / L 犦 (208.8 ± 17.2) ng / (222.9 ± 16.5) ng / L L in L 犦 and O + P + E groups were significantly lower than those in OVX group (336.6 ± 18.1) ng / L 犦 (t = 11.160, 7.783 and 14.971, P <0.01). CONCLUSION: Propranolol can improve the bone mineral density of osteoporotic rats after ovariectomy, and can effectively prevent the bone loss and reduce the serum level of OVI rats.