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用免疫组织化学方法研究RCS大鼠(Royal College of Surgeon’s rat,RCS rat)视网膜变性过程中早、中、晚期二级神经元形态的变化。结果发现,P1MRCS大鼠各二级神经元未见明显的形态改变。P2M RCS大鼠视网膜外核层萎缩约85%,视杆双极细胞顶端树突萎缩;水平细胞的树突与轴突在外网状层的分层未见改变。P3M RCS大鼠视网膜外核层萎缩近95%,RCS大鼠视杆双极细胞顶端萌生新的神经突起;水平细胞的树突分支明显丢失,轴突在外网状层的分层发生改变,出现新生神经突起;无长突细胞的树突在内网状层的分层至变性晚期也未见改变。该研究表明RCS大鼠视网膜二级神经元形态的改变是继发性改变,是感光细胞变性后对传入缺失的一种反应,即重构反应。在进行视网膜功能救治时需要考虑重构反应带来的影响。
Immunohistochemistry was used to study the morphological changes of early, middle and late secondary neurons during the retinal degeneration of RCS rats (RCS rat). The results showed that, P1MRCS rat secondary neurons no obvious morphological changes. P2M RCS rat retinal outer nuclear layer atrophy about 85%, rod bipolar apical dendrites atrophy; horizontal dendrites and axons in the outer reticular layer stratification was not changed. In the P3M RCS rats, the retinal outer nuclear layer atrophied by nearly 95%, new neurites in the top of rod-like bipolar cells of RCS rats appeared; the dendritic branches of horizontal cells were obviously lost and the axons changed in the delamination of the outer reticular layer Neonatal neurite; dendritic cells without dendrites within the reticular delamination stratification to late degeneration has not changed. The study shows that RCS rat secondary retinal neuronal morphology is a secondary change is degeneration of photoreceptor cells on the introduction of a response to the lack of response, that is, the remodeling reaction. In the treatment of retinal function need to consider the impact of remodeling reaction.