论文部分内容阅读
小肠结肠炎耶尔森菌的毒力质粒可表达外膜蛋白YadA和分泌蛋白YopS,能抑制人粒细胞对小肠结肠炎耶尔森菌的吞噬和杀伤调理作用。作者为阐明YopS在耶尔森菌与粒细胞之间的相互作用。阐明确保YadA抑或YopS影响人粒细胞吞噬和杀伤调理作用,用正常人粒细胞和携带毒力质粒(PYV~+)与无毒力质粒(PYV~-)小肠结肠炎耶尔森菌进行生存率、吞噬率及杀伤率的研究。研究结果表明经计算和直线回归分析,加有兔免疫血清的粒细胞悬液,经90min孵育,PYV~-和PYV~+菌数减少分别为3.23±
Yersinia enterocolitica virulence plasmid can express the outer membrane protein YadA and secretory protein YopS, can inhibit human granulocyte phagocytosis and killing effect on Yersinia enterocolitica. To elucidate the interaction of YopS between Yersinia and granulocytes. To clarify whether YadA or YopS can affect the phagocytosis of human granulocytes and the killing effect, the survival rate of Yersinia enterocolitica with normal human granulocytes and virulence plasmid (PYV ~ +) and virulence plasmid (PYV ~ -) , Phagocytosis rate and killing rate of the study. The results showed that by calculating and linear regression analysis, adding rabbit immune serum granulocyte suspension, after 90min incubation, PYV ~ - and PYV ~ + bacteria decreased by 3.23 ±