大鼠固定(每天2.5h)连续7天,用放免法分别测得固定前1天及固定1、3、5、7天血浆皮质酮含量。结果发现,随着固定的持续,血浆皮质酮的含量逐渐升高,固定第3天即达高峰60.4±5.0μg/dl(P<0.01)以后有所下降,固定第5天为56±3.4μg/dl(P<0.01),第7天为47.3±2.5μg/dl(P<0.01)。而对照组在相同期间皮质酮含量前后对比无显著性差异。这一结果表明,在固定应激过程中,下丘脑—垂体—肾上腺皮质系统起了一定的作用。 Rats were fixed (2.5h daily) for 7 consecutive days. The levels of plasma corticosterone were measured by radioimmunoassay 1 day before and 1, 3, 5 and 7 days. The results showed that plasma corticosterone level increased gradually with the duration of immobilization and decreased after reaching the peak value of 60.4 ± 5.0μg / dl (P <0.01) on the third day of immobilization, with 56 ± 3.4μg on the fifth day / dl (P <0.01) and 47.3 ± 2.5 μg / dl on day 7 (P <0.01). The control group in the same period before and after corticosterone content was no significant difference. This result suggests that the hypothalamic-pituitary-adrenal system plays a role in the stress-fixed process.