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为研究缺铁性贫血（ＩＤＡ）对大鼠学习记忆能力的影响及其生化基础，应用低铁饮食（含铁量为１１．９ｍｇ／ｋｇ饲料）建立了ＩＤＡ大鼠模型，用ＭＧ－２型３等分辐射式迷宫检测了大鼠的学习记忆能力，用３电极等离子体原子发射直读光谱仪测定脑组织铁含量，酶组化法测定纹状体单胺氧化酶（ＭＡＯ）活性，高效液相色谱电化学检测器测定大脑皮层及海马组织中单胺类神经递质及其代谢产物的含量。结果显示ＩＤＡ大鼠在迷宫测验中达标所需反应次数显著多于对照组，正确反应率显著低于对照组，脑组织铁含量及ＭＡＯ活性均降低，大脑皮层去甲肾上腺素（ＮＥ）及５－羟色胺（５－ＨＴ）含量较对照组升高，海马组织中ＮＥ含量也升高，５－ＨＴ的代谢产物５－羟吲哚乙酸（５－ＨＩＡＡ）较对照组降低。结果表明，ＩＤＡ大鼠的学习记忆能力明显降低，脑组织单胺类神经递质的代谢异常是ＩＤＡ大鼠学习记忆障碍的生化基础之一。
To investigate the effect of IDA on learning and memory in rats and its biochemical basis, a rat model of IDA was established by low iron diet (iron content 11.9mg / kg diet) 3 rats were examined by isotonic radiative maze for learning and memory. The iron content in brain tissue was measured by direct-reading three-electrode plasma atomic emission spectroscopy. The activity of monoamine oxidase (MAO) in striatum was determined by enzyme histochemistry. The activity of high performance liquid chromatography Chemical detector for the determination of monoamine neurotransmitters and their metabolites in the cerebral cortex and hippocampus. The results showed that IDA rats in the maze test to reach the desired number of reactions significantly more than the control group, the correct response rate was significantly lower than the control group, brain tissue iron content and MAO activity were decreased, cortical norepinephrine (NE) and 5 The level of 5-hydroxytryptamine (5-HIAA) in hippocampus was also increased compared with the control group. The 5-HIAA metabolite of 5-HT was lower than that of the control group. The results showed that learning and memory abilities of IDA rats were significantly reduced, and the abnormal metabolism of monoamine neurotransmitters in brain tissue was one of the biochemical basis of learning and memory impairment in IDA rats.