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目的:建立同时测定裸花紫珠药材中木犀草素-7-O-β-D-吡喃葡萄糖苷、木犀草素、5,4’-二羟基-3,7,3’-三甲氧基黄酮和5-羟基-3,7,3’,4’-四甲氧基黄酮含量的反相高效液相色谱法。方法:采用HPLC,Venusil XBP-C18色谱柱(4.6 mm×200mm,5μm),流动相甲醇-体积分数为0.1%磷酸水系统,梯度洗脱,流速1.0 mL·min﹣1,柱温室温,检测波长350 nm。结果:在上述色谱条件下测得木犀草素-7-O-β-D-吡喃葡萄糖苷、木犀草素、5,4’-二羟基-3,7,3’-三甲氧基黄酮和5-羟基-3,7,3’,4’-四甲氧基黄酮的质量浓度分别在0.000 8~0.016,0.001 6~0.032,0.004~0.08,0.002 4~0.048 g·L-1时与色谱峰面积之间线性关系良好;平均回收率为98.5%(RSD 1.7%),98.6%(RSD 1.6%),98.6%(RSD 1.4%),98.6%(RSD 1.7%)。结论:该方法精密度高,重复性好,可用于裸花紫珠药材中木犀草素-7-O-β-D-吡喃葡萄糖苷、木犀草素、5,4’-二羟基-3,7,3’-三甲氧基黄酮和5-羟基-3,7,3’,4’-四甲氧基黄酮的含量测定。
Objective: To establish a simultaneous determination of luteolin-7-O-β-D-glucopyranoside, luteolin, 5,4’-dihydroxy-3,7,3’-trimethoxy Flavonoids and 5-hydroxy-3,7,3 ’, 4’-tetramethoxyflavone content of the RP-HPLC. Methods: The mobile phase was eluted with a Venusil XBP-C18 column (4.6 mm × 200 mm, 5 μm) using a mobile phase of methanol-0.1% phosphoric acid with gradient elution at a flow rate of 1.0 mL · min-1. Wavelength 350 nm. Results: Luteolin-7-O-β-D-glucopyranoside, luteolin, 5,4’-dihydroxy-3,7,3’-trimethoxyflavone were measured under the above chromatographic conditions and The mass concentration of 5-hydroxy-3,7,3 ’, 4’-tetramethoxyflavone was 0.0008 ~ 0.016,0.001 6 ~ 0.032,0.004 ~ 0.08,0.002 4 ~ 0.048 g · L -1 and the chromatogram The linear relationship between peak areas was good. The average recoveries were 98.5% (RSD 1.7%), 98.6% (RSD 1.6%), 98.6% (RSD 1.4%) and 98.6% (RSD 1.7%). Conclusion: The method has high precision and good reproducibility. It can be used in luteolin -7-O-β-D-glucopyranoside, luteolin, 5,4’-dihydroxy-3 , 7,3’-trimethoxyflavone and 5-hydroxy-3,7,3 ’, 4’-tetramethoxyflavone.