采用RT-PCR法扩增出中国境内生长的琉璃苣Δ6-脂肪酸脱氢酶基因,序列分析表明,该基因全长为1 359个核苷酸,与美国德克萨斯州的琉璃苣Δ6-脂肪酸脱氢酶基因同源性可达到99%。将克隆到的基因连接pRTL2质粒的35S启动子,构建重组载体pPTN-D6D,并通过农杆菌介导的子叶节转化系统,将该基因导入到垦农18和小粒豆2个大豆品种中。经PCR检测分析,初步证明琉璃苣Δ6-脂肪酸脱氢酶基因已导入并整合到大豆基因组中。 The gene of Δ6-fatty acid dehydrogenase of borage grown in China was amplified by RT-PCR. The sequence analysis showed that the gene was 1 359 nucleotides in length, which was identical to that of the American borage Δ6- Fatty acid dehydrogenase gene homology can reach 99%. The cloned gene was ligated to the 35S promoter of pRTL2 plasmid to construct the recombinant vector pPTN-D6D. The gene was introduced into Kenong 18 and two small soybean cultivars through agrobacterium-mediated cotyledonary node transformation system. The results of PCR analysis showed that the borage Δ6-fatty acid dehydrogenase gene was introduced and integrated into soybean genome.