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人体和实验动物原发性和继发性肝癌的血供主要来自肝动脉,间歇阻断肝动脉1/2～2小时已用作有效的治疗方法,取Wistar雄鼠作肝动脉阻断后的分子生物学观察.将含1.0×10~6二甲基肼诱发的结肠癌活细胞混悬液直接种植至动物的肝左外侧叶,5天后在肝动脉周围置微阻断器,并结扎输入肝脏的侧支循环,取4只鼠作对照,次日开始阻断肝动脉,计30分钟、60分钟和120分钟,每组各4只鼠;测定肿瘤组织 RNA(mg/mg DNA)和各种核苷酸(nmol/mg DNA),如尿苷三磷酸(UTP)、鸟苷三磷酸(GTP)、尿苷二磷酸(UDP)、腺苷三磷酸(ATP)、腺苷二磷酸(ADP)、腺苷一磷酸(AMP)和辅酶1(NAD).在实验二中,观察再灌流后的变化,取5只鼠作为对照,阻断30分钟后复流2小时(5只鼠)、阻断2小时(5只鼠)、阻断2小时后复流2小时(2只鼠)、阻断2小时后复流22小时(6只鼠)和阻断2小时后复流40小时(2只鼠),分别作实验一的各项测定.
The blood supply of primary and secondary liver cancers in humans and experimental animals is mainly from the hepatic artery. Intermittent blocking of the hepatic artery for 1/2 to 2 hours has been used as an effective treatment method. Wistar male rats were used for hepatic artery occlusion. Molecular biology observation. Live cells suspension containing 1.0×10 6 dimethyl hydrazine-induced colon cancer was directly implanted into the left lateral lobe of the animal. After 5 days, a micro blocker was placed around the hepatic artery and the ligation was input. In the collateral circulation of the liver, 4 rats were used as controls, and the hepatic artery was blocked on the following day for 30 minutes, 60 minutes, and 120 minutes, with 4 mice in each group; RNA (mg/mg DNA) and each of the tumor tissues were determined. Nucleotides (nmol/mg DNA) such as uridine triphosphate (UTP), guanosine triphosphate (GTP), uridine diphosphate (UDP), adenosine triphosphate (ATP), adenosine diphosphate (ADP) ), adenosine monophosphate (AMP) and coenzyme 1 (NAD). In experiment 2, changes after reperfusion were observed, and 5 rats were used as controls to block reflow for 2 hours (5 mice) after 30 minutes. Blocked for 2 hours (5 mice), blocked 2 hours after reflow 2 hours (2 rats), blocked 2 hours after reflow 22 hours (6 rats), and blocked 2 hours after reflow 40 hours ( 2 rats), respectively, for the determination of the experimental one.